Anti-alpha-Smooth Muscle Actin antibody (monoclonal)

Product code: MA1106 Boster Citations: 142 Datasheet Protocols
Price: $200.00


Product Name Anti-alpha-Smooth Muscle Actin antibody (monoclonal)
Description Mouse IgG monoclonal antibody for alpha-Smooth Muscle Actin, actin, alpha 2, smooth muscle, aorta (ACTA2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.
Species Human, Mouse, Rat
Application IHC-P, IHC-F, WB
Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
Immunogen N-terminal synthetic decapeptide of alpha-smooth muscle actin.
Specificity No cross reactivity with other proteins
Pack Size 100μg/vial


Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites

Application Details

Application Concentration* Species Validated Using**
Western blot2μg/mlHuman, mouse, rat.Boster's ECL kit
Immunohistochemistry(Paraffin-embedded Section)0.1-0.5μg/mlHuman, mouse, rat.Boster's SuperVision kit
Immunohistochemistry(Frozen Section)0.1-0.5μg/mlHuman, mouse, rat.Boster's SuperVision kit
*Optimal dilutions should be determined by users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.

Related products

Protein Target Info (Source:

Gene Name 1867
Protein Name Actin, aortic smooth muscle
Gene Full Name actin, alpha 2, smooth muscle, aorta
Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Localization Cytoplasm, cytoskeleton.
Uniprot ID Human(P62736), Mouse(P62737), Rat(P62738)
Alternative Names Actin, aortic smooth muscle|ACTA2|Actin,aorticsmoothmuscle
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Actin, aortic smooth muscle (1867)

Ueyama et al.(1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.


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