Anti-Serine-protein kinase ATM ATM Antibody

ATM antibody

Boster Bio Anti-Serine-protein kinase ATM ATM Antibody catalog # RP1040. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: RP1040
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Serine-protein kinase ATM ATM Antibody

View all ATM Antibodies

SKU/Catalog Number

RP1040

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Serine-protein kinase ATM ATM Antibody catalog # RP1040. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Serine-protein kinase ATM ATM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1040)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ATM recombinant protein (Position: D2870-V3056). Human ATM shares 95% amino acid (aa) sequence identity with mouse ATM.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1040 is reactive to ATM in Human

Applications

RP1040 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

350.687kDa

Background of ATM

ATM (ataxia telangiectasia mutated), also known as TEL1 or TELO1, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. The ATM gene is mapped to chromosome 11q22.3. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not as important for the proliferation or differentiation of progenitors in a telomere-independent manner. ATM functions directly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA ends in repair complexes generated during lymphocyte gene assembly.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For ATM (Source: Uniprot.org, NCBI)

Gene Name

ATM

Full Name

Serine-protein kinase ATM

Weight

350.687kDa

Superfamily

PI3/PI4-kinase family

Alternative Names

AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutatedATD; ATC; ATDC; ATE; ATM; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL ATM AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1 ATM serine/threonine kinase serine-protein kinase ATM|A-T mutated|AT mutated|TEL1, telomere maintenance 1, homolog|ataxia telangiectasia mutated

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATM, check out the ATM Infographic

ATM infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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14 Customer Q&As for Anti-Serine-protein kinase ATM ATM Antibody

Question

See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-ATM antibody RP1040. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-24

Question

I see that the anti-ATM antibody RP1040 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-13

Question

We are interested in to test anti-ATM antibody RP1040 on human cervix carcinoma for research purposes, then I may be interested in using anti-ATM antibody RP1040 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

The products we sell, including anti-ATM antibody RP1040, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-26

Question

Will anti-ATM antibody RP1040 work for WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

According to the expression profile of cervix carcinoma, ATM is highly expressed in cervix carcinoma. So, it is likely that anti-ATM antibody RP1040 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2020-02-06

Question

My boss were well pleased with the WB result of your anti-ATM antibody. However we have observed positive staining in fibroblast nucleus using this antibody. Is that expected? Could you tell me where is ATM supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

From literature, fibroblast does express ATM. Generally ATM expresses in nucleus. Regarding which tissues have ATM expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 7792600
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-11-13

Question

I was wanting to use your anti-ATM antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

It shows on the product datasheet, RP1040 anti-ATM antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-30

Question

We are currently using anti-ATM antibody RP1040 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

R. Jones

Verified customer

Asked: 2019-09-24

Answer

The anti-ATM antibody (RP1040) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-24

Question

Is this RP1040 anti-ATM antibody reactive to the isotypes of ATM?

Verified Customer

Verified customer

Asked: 2019-04-30

Answer

The immunogen of RP1040 anti-ATM antibody is E.coli-derived human ATM recombinant protein (Position: D2870-V3056). Human ATM shares 95% amino acid (aa) sequence identity with mouse ATM. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-04-30

Question

We have seen staining in human corpus callosum. Any tips? Is anti-ATM antibody supposed to stain corpus callosum positively?

Verified Customer

Verified customer

Asked: 2019-04-12

Answer

From literature corpus callosum does express ATM. From Uniprot.org, ATM is expressed in corpus callosum, fibroblast, brain, embryonic kidney, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ATM expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 7792600
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-04-12

Question

My question regarding product RP1040, anti-ATM antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-12-28

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1040 anti-ATM antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-28

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-ATM antibody RP1040. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-12-04

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-04

Question

Is a blocking peptide available for product anti-ATM antibody (RP1040)?

Verified Customer

Verified customer

Asked: 2017-11-20

Answer

We do provide the blocking peptide for product anti-ATM antibody (RP1040). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-11-20

Question

Does RP1040 anti-ATM antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

R. Carter

Verified customer

Asked: 2017-07-19

Answer

You can see on the product datasheet, RP1040 anti-ATM antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-07-19

Question

Is there a BSA free version of anti-ATM antibody RP1040 available?

N. Jha

Verified customer

Asked: 2017-05-10

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ATM antibody RP1040 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-05-10

Order DetailsPrice
RP1040

100μg

$370
RP1040-10ug

10μg sample (liquid)

$99
RP1040-Biotin

100 μg Biotin conjugated

$570
RP1040-Cy3

100 μg Cy3 conjugated

$570
RP1040-Dylight488

100 μg Dylight488 conjugated

$570
RP1040-Dylight550

100 μg Dylight550 conjugated

$570
RP1040-Dylight594

100 μg Dylight594 conjugated

$570
RP1040-FITC

100 μg FITC conjugated

$570
RP1040-HRP

100 μg HRP conjugated

$570
RP1040-APC

100 μg APC conjugated

$670
RP1040-PE

100 μg PE conjugated

$670
RP1040-carrier-free

Carrier Free

$370

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RP1040
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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