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SKUAR0001
Size10 mL (sufficient for coating 100-200 slides)
Physical StateLiquid
ApplicationTo treat the glass slide before mounting tissue sections during immunohistochemistry (IHC)
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Overview

Product Name (APTES)3-Triethoxysilylpropylamine
SKU/Catalog Number AR0001
Form Supplied Concentrated solution (50X)
Physical State Liquid
Storage Upon receipt store at 4°C. (APTES)3-Triethoxysilylpropylamine is stable at 4°C for one year.
Pack Size 10 mL (sufficient for coating 100-200 slides)
Dilution Ratio 1:50
Equivalent Millipore Sigma (Product No. A3648)
Toxicity APTES is a toxic compound. APTES fumes are destructive to the mucous membranes and the upper respiratory tract, and should be used in a fume hood with gloves. If a fume hood is not available, a face shield and full face respirator must be implemented.
Application To treat the glass slide before mounting tissue sections during immunohistochemistry (IHC)
*Our Boster Guarantee covers the use of this product in the above tested applications.
Cite This Product (APTES)3-Triethoxysilylpropylamine (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0001)

Chemical structures

Molecular formula: H2N(CH2)3Si(OC2H5)3

Molecular weight: 221.37

CAS Number: 919-30-2

Description

(APTES)3-Aminopropyltriethoxysilane is a silylation agent used to treat glass slides and create a reactive film (primary Amines). Treated surface can be used to crosslink and immobilize proteins and other molecules.
APTES is used to treat the glass slide before mounting tissue sections during immunohistochemistry (IHC).

Procedure

1. Dilute (APTES)3-Aminopropyltriethoxysilane 1:50 with acetone in a plastic beaker.

2. Fully coat clean slides with diluted (APTES)3-Aminopropyltriethoxysilane solution. A rack at a time and incubate for 2 minutes at room temperature.

3. Wash the slides with distilled water 1-2 times.

4. Aspirate the (APTES)3-Aminopropyltriethoxysilane solution, drain slides and dry in 60°C oven for 1 hour.

Notes

1. Dilute (APTES)3-Aminopropyltriethoxysilane should be freshly prepared before use.

2. There will be a small amount of milk-white substance on the slide after coating.

(APTES)3-Triethoxysilylpropylamine Images

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(APTES)3-Triethoxysilylpropylamine
Chemical structure
(APTES)3-Triethoxysilylpropylamine
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Publications

You Y, Huan P, Liu B. Mol Biol Rep. 2012 Aug;39(8):8209-16. Doi: 10.1007/S11033-012-1668-Y. Epub 2012 Apr 28. Rnai Assay In Primary Cells: A New Method For Gene Function Analysis In Marine Bivalve.
Lu H, Wang Ss, Wang Wl, Zhang L, Zhao By. J Agric Food Chem. 2014 Jul 30;62(30):7407-12. Doi: 10.1021/Jf501299D. Epub 2014 Apr 17. Effect Of Swainsonine In Oxytropis Kansuensis On Golgi ??-Mannosidase Ii Expression In The Brain Tissues Of Sprague-...
Yu Y, Lv N, Lu Z, Zheng Yy, Zhang Wc, Chen C, Peng Yj, He Wq, Meng Fq, Zhu Ms, Chen Hq. Febs J. 2012 Apr;279(8):1485-94. Doi: 10.1111/J.1742-4658.2012.08541.X. Epub 2012 Mar 16. Deletion Of Myosin Light Chain Kinase In Endothelial Cells Has A Mino...