|Size||10 mL (sufficient for coating 100-200 slides)|
|Application||To treat the glass slide before mounting tissue sections during immunohistochemistry (IHC)|
Data & Images
|Form Supplied||Concentrated solution (50X)|
|Storage||Upon receipt store at 4°C. (APTES)3-Triethoxysilylpropylamine is stable at 4°C for one year.|
|Pack Size||10 mL (sufficient for coating 100-200 slides)|
|Equivalent||Millipore Sigma (Product No. A3648)|
|Toxicity||APTES is a toxic compound. APTES fumes are destructive to the mucous membranes and the upper respiratory tract, and should be used in a fume hood with gloves. If a fume hood is not available, a face shield and full face respirator must be implemented.|
|Application||To treat the glass slide before mounting tissue sections during immunohistochemistry (IHC)
*Our Boster Guarantee covers the use of this product in the above tested applications.
|Cite This Product||(APTES)3-Triethoxysilylpropylamine (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0001)|
Molecular formula: H2N(CH2)3Si(OC2H5)3
Molecular weight: 221.37
CAS Number: 919-30-2
(APTES)3-Aminopropyltriethoxysilane is a silylation agent used to treat glass slides and create a reactive film (primary Amines). Treated surface can be used to crosslink and immobilize proteins and other molecules.
APTES is used to treat the glass slide before mounting tissue sections during immunohistochemistry (IHC).
1. Dilute (APTES)3-Aminopropyltriethoxysilane 1:50 with acetone in a plastic beaker.
2. Fully coat clean slides with diluted (APTES)3-Aminopropyltriethoxysilane solution. A rack at a time and incubate for 2 minutes at room temperature.
3. Wash the slides with distilled water 1-2 times.
4. Aspirate the (APTES)3-Aminopropyltriethoxysilane solution, drain slides and dry in 60°C oven for 1 hour.
1. Dilute (APTES)3-Aminopropyltriethoxysilane should be freshly prepared before use.
2. There will be a small amount of milk-white substance on the slide after coating.
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