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SKU:AR1068
Size:500ml, ready-to-use
Application:Preserving Samples, Fixing Cells For Histology
Description:4% Paraformaldehyde solution in PBS is a ready-to-use solution for sample preparation and fixing cells for immunohistochemistry (IHC).
Price: $50.00
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Overview

Product Name 4% Paraformaldehyde (PFA) Solution in PBS
Description 4% Paraformaldehyde solution in PBS is a ready to use solution used for sample preparation for fixing cells for immunohistochemistry (IHC)
Cite This Product 4% Paraformaldehyde (PFA) Solution in PBS (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1068)
Pack Size 500ml
Safety and Handling Causes serious eye damage.
Suspected of causing cancer.
May cause an allergic skin reaction.
Causes skin irritation.
Avoid breathing dust/fume/gas/mist/vapors/spray.
Wear protective gloves/protective clothing/eye protection/face protection.
Use personal protective equipment as required.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Store locked up.
Dispose of contents/container in accordance with local/regional/national/international regulations.

Properties

StorageStore at -20˚C.
CAS Number30525-89-4
MDL NumberMFCD00133991
FormLiquid
Physical State The solution should be clear, colorless, with no precipitate.
Solubility Soluble in water
pH 7.0 - 7.6 at 25°C

Background

Paraformaldehyde (PFA) is the polymerization product of formaldehyde with a typical degree of polymerization of 8–100 units. Paraformaldehyde is not a fixative itself; it must be depolymerized to formaldehyde in solution. In cell culture, a typical formaldehyde fixing procedure would involve using a 4% formaldehyde solution in phosphate buffered saline (PBS) on ice for 10 minutes. Fixing ensures that sample cell structures stay intact and that antigens are immobilized, while ideally still permitting unfettered access of antibodies to target antigens.

4% Paraformaldehyde (PFA) Solution in PBS Images

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4% Paraformaldehyde (PFA) Solution in PBS
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Publications

Cheng, B., Zheng, H., Wu, F., Wu, J., Liu, X., Tang, C.,..., & Su, Z. (2017). Metabolomics analysis of Danggui Sini decoction on treatment of collagen-induced arthritis in rats. Journal of Chromatography B, 1061-1062, 282-291. doi: 10.1016/j.jchromb.2017.07.043
Mei, F., Wang, J.G., Chen, Z.J., & Yuan, Z.L. (2017). Effects of epoxyeicosatrienoic acids (EETs) on retinal macular degeneration in rat models. European Review for Medical and Pharmacological Sciences, 21(12), 2970-2979. Retrieved from http://www.europeanreview.org/article/12960
Liu, X., Li, Y., He, J., Zhao, T., Chen, C., Gu, H., & Wang, X. (2017). Paclitaxel-loaded pluronic F127/P123 silica nanocapsules with surface conjugated rhTRAIL for targeted cancer therapy. RSC Advances, 7(48), 30250–30261. Advance online publication. doi: 10.1039/c7ra04503d
Ren, J., Lin, L., Wang, X., Cai, Z., Li, C., Li, D., & Chang, Q. (2017). Role of Chloride Channel-3 (ClC-3) in SIN-1-induced apoptosis in rat hippocampal neurons. Biomedical Research, 28(9), 3991-3995. Retrieved from http://www.alliedacademies.org/articles/role-of-chloride-channel3-clc3-in-sin1induced-apoptosis-in-rat-hippocampal-neurons.pdf
Liu Q, Long Z, Dong X, Zhang T, Zhao J, Sun B, Zhu J, Li J, Wang Q, Yang Z, Hu X, Li L. Exp Mol Med. 2017 Apr 21;49(4):e319. doi: 10.1038/emm.2017.31. Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.
Wang J, Yang Z, Wen J, Ma F, Wang F, Yu K, Tang M, Wu W, Dong Y, Cheng X, Nie C, Chen L. J Pharmacol Sci. 2014;126(3):198-207. Epub 2014 Oct 21. Sklb-M8 Induces Apoptosis Through The Akt/Mtor Signaling Pathway In Melanoma Models And Inhibits Angio...
Wang Q, Zhu J, Zou L, Yang Y. J Huazhong Univ Sci Technolog Med Sci. 2011 Apr;31(2):246-50. Doi: 10.1007/S11596-011-0261-2. Epub 2011 Apr 20. Role Of Axonal Guidance Factor Netrin-1 In Human Placental Vascular Growth.
Zhao Jw, Gao Zl, Mei H, Li Yl, Wang Y. Am J Med Sci. 2011 Jun;341(6):460-8. Doi: 10.1097/Maj.0B013E31820865D5. Differentiation Of Human Mesenchymal Stem Cells: The Potential Mechanism For Estrogen-Induced Preferential Osteoblast Versus Adipocyte D...
Zhang P, Wu X, Hu C, Wang P, Li X. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):30-6. Doi: 10.1007/S11626-011-9471-Y. Epub 2011 Dec 17. Rho Kinase Inhibitor Y-27632 And Accutase Dramatically Increase Mouse Embryonic Stem Cell Derivation.
Sun Y, Chen S, Song G, Ren L, Wei L, Liu N, Zhang D, Lv X. Int J Mol Med. 2012 Sep;30(3):622-8. Doi: 10.3892/Ijmm.2012.1032. Epub 2012 Jun 14. Effect Of Visfatin On The Function Of Endothelial Progenitor Cells In High-Fat-Fed Obese Rat...
Wang Y, Li Jr, Sun Mx, Ni B, Huan C, Huang L, Li C, Fan Hj, Ren Xf, Mao X. Antiviral Res. 2014 Jun;106:33-41. Doi: 10.1016/J.Antiviral.2014.03.007. Epub 2014 Mar 25. Triggering Unfolded Protein Response By 2-Deoxy-D-Glucose Inhibits Porcine Epidem...
Liu C, Xia X, Miao W, Luan X, Sun L, Jin Y, Liu L. Int J Pharm. 2013 Oct 15;455(1-2):312-9. Doi: 10.1016/J.Ijpharm.2013.07.004. Epub 2013 Jul 15. An Ovarian Cell Microcapsule System Simulating Follicle Structure For Providing Endogenous Female Hor...
Jin F, Li L, Shi M, Li Z, Zhou J, Chen L. Behav Brain Res. 2013 Jun 1;246:116-24. Doi: 10.1016/J.Bbr.2013.02.029. Epub 2013 Mar 15. The Longitudinal Study Of Rat Hippocampus Influenced By Stress: Early Adverse Experience Enhances Hippocampal Vulne...
Chen F, Wang H, Xiang X, Yuan J, Chu W, Xue X, Zhu H, Ge H, Zou M, Feng H, Lin J. J Surg Res. 2014 Dec;192(2):298-304. Doi: 10.1016/J.Jss.2014.06.026. Epub 2014 Jun 19. Curcumin Increased The Differentiation Rate Of Neurons In Neural Stem Cells Vi...
Wang J, Ren Y, Guo X, Cheng H, Ye Y, Qi J, Yang C, You H. Mol Med Rep. 2015 May;11(5):3585-92. Doi: 10.3892/Mmr.2015.3164. Epub 2015 Jan 8. Alterations In Enhancer Of Zeste Homolog 2, Matrix Metalloproteinase-2 And Tissue Inhibitor Of Metalloprote...
Zhang R, Fang H, Chen Y, Shen J, Lu H, Zeng C, Ren J, Zeng H, Li Z, Chen S, Cai D, Zhao Q. Plos One. 2012;7(2):E32356. Doi: 10.1371/Journal.Pone.0032356. Epub 2012 Feb 27. Gene Expression Analyses Of Subchondral Bone In Early Experimental Osteoart...
Pang W, Zhang Y, Zhao N, Darwiche Ss, Fu X, Xiang W. Placenta. 2013 Jul;34(7):613-8. Doi: 10.1016/J.Placenta.2013.03.013. Epub 2013 Apr 17. Low Expression Of Mfn2 Is Associated With Mitochondrial Damage And Apoptosis In The Placental Villi Of Earl...
Peterson Am, Pilz-Allen C, Kolesnikova T, M??hwald H, Shchukin D. Acs Appl Mater Interfaces. 2014 Feb 12;6(3):1866-71. Doi: 10.1021/Am404849Y. Epub 2013 Dec 16. Growth Factor Release From Polyelectrolyte-Coated Titanium For Implant Applications.
Xing J, Jin H, Hou T, Chang Z, Luo F, Wang P, Li Z, Xie Z, Xu J. J Surg Res. 2014 Dec;192(2):454-63. Doi: 10.1016/J.Jss.2014.05.037. Epub 2014 May 27. Establishment Of A Bilateral Femoral Large Segmental Bone Defect Mouse Model Potentially Applica...
Ding Y, Yuan S, Liu X, Mao P, Zhao C, Huang Q, Zhang R, Fang Y, Song Q, Yuan D, Xie P, Liu Y, Liu Q. Plos One. 2014 Nov 20;9(11):E112207. Doi: 10.1371/Journal.Pone.0112207. Ecollection 2014. Protective Effects Of Astragaloside Iv On Db/Db Mice Wit...
Lv Y, Xia Jy, Chen Jy, Zhao H, Yan Hc, Yang Hs, Li Q, Fan Yx, Guo Kj, Chen Xy. Bmc Musculoskelet Disord. 2014 Nov 6;15:370. Doi: 10.1186/1471-2474-15-370. Effects Of Pamidronate Disodium On The Loss Of Osteoarthritic Subchondral Bone And The Expre...
Liang Gb, Luo Gh, Bao Ds, Chen Aj, Zhuang Yx, Guo Yn, Wang X, Wang Yl, Chen Zp, Lu Yp, Li Yp. Mol Med Rep. 2015 Aug;12(2):2577-83. Doi: 10.3892/Mmr.2015.3713. Epub 2015 May 4. Impact Of Immunosuppressive Agents On The Expression Of Indoleamine 2,3...
Han L, Wu C, Riaz H, Bai L, Chen J, Zhen Y, Guo A, Yang L. Plos One. 2013 Oct 3;8(10):E74596. Doi: 10.1371/Journal.Pone.0074596. Ecollection 2013. Characterization Of The Mechanism Of Inhibin ??-Subunit Gene In Mouse Anterior Pituitary Cells By Rna...
Liu C, Huang Y, Pang M, Yang Y, Li S, Liu L, Shu T, Zhou W, Wang X, Rong L, Liu B. Plos One. 2015 Mar 24;10(3):E0117709. Doi: 10.1371/Journal.Pone.0117709. Ecollection 2015. Tissue-Engineered Regeneration Of Completely Transected Spinal Cord Using...
Gu X, Fu J, Bai J, Zhang C, Wang J, Pan W. Am J Med Sci. 2015 Feb;349(2):157-61. Doi: 10.1097/Maj.0000000000000385. Low-Frequency Electrical Stimulation Induces The Proliferation And Differentiation Of Peripheral Blood Stem Cells Into Schwann Cells.

FAQs

Q: How long can this product be stored after being opened at -20°C?
A: Please use this product within the shelf life of one year at -20°C after the solution is opened
Q: Can I dilute AR1068 from 4% to 1% Paraformaldehyde (PFA) solution in PBS? What is the protocol? Keywords: dilution, concentration
A: Yes, the 4% Paraformaldehyde can be diluted to 1% in PBS. The dilution protocol is as follows: (1) Prepare the PBS by adding 14.2g Na2HPO4 and 9g NaCl into 1000ml H2O. (2) Mix 500mL 4% Paraformaldehyde with 1500mL PBS thoroughly.
Q: How pure (%) is the PFA in your pre-made solution?
A: Purity of the solid formaldehyde is ≥95%.
Q: We will use this to fix bacterial cells from faecal samples. Do you have any reference for this use?
A: We haven't use AR1068 to fix bacterial cells from faecal samples. We could not provide any reference for this use. We're sorry for the inconvenience.
Q: Since we will be using this on a daily basis, should it be stored at room temperature or at 4°C? What is the shelf life for these conditions?
A: Please store the product at 4°C if it is frequently used. The shelf life is 3 months at 4°C. For long storage, please store at -20°C.
Q: Is this product DNA/RNA free?
A: Yes, AR1068 is DNA/RNA free.
Q: What is the PBS concentration?
A: PBS concentration is 0.01g/mL.
Q: What is the detailed composition of AR1068?
A: AR1068 contains paraformaldehyde, Na2HPO4, NaCl, and DEPC.
Q: Is the 4% PFA solution in PBS methanol free?
A: Yes, it is methanol free.