ADCK4 Colorimetric Cell-Based ELISA

COQ8B ELISA kit, cell-based

The ADCK4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ADCK4 protein expression profile in cells. The kit can be used for measuring the relative amounts of ADCK4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ADCK4.

Product Info Summary

SKU: EKC1897
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

ADCK4 Colorimetric Cell-Based ELISA

See all COQ8B products

SKU/Catalog Number

EKC1897

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The ADCK4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ADCK4 protein expression profile in cells. The kit can be used for measuring the relative amounts of ADCK4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ADCK4.

Storage & Handling

4°C/6 Months

Cite This Product

ADCK4 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1897)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1897 is reactive to COQ8B ADCK4 in Human, Mouse, Rat samples

Application Guarantee

EKC1897 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-COQ8B Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For COQ8B ADCK4 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q96D53

Gene ID

79934

Gene Name

COQ8B ADCK4

Full Name

Atypical kinase COQ8B, mitochondrial

Weight

60.069kDa

Superfamily

protein kinase superfamily

Alternative Names

Atypical kinase COQ8B, mitochondrial COQ8B ADCK4, NPHS9 coenzyme Q8B atypical kinase COQ8B, mitochondrial|aarF domain containing kinase 4|aarF domain-containing protein kinase 4|coenzyme Q protein 8B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on COQ8B ADCK4 , check out the COQ8B ADCK4 Infographic

COQ8B ADCK4  infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for COQ8B ADCK4 : database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

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Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

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