Akt1 (Phospho-Thr308) Colorimetric Cell-Based ELISA Kit

The Akt1 (Phospho-Thr308) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Akt1 (Phospho-Thr308) protein expression profile in cells. The kit can be used for measuring the relative amounts of Akt1 (Phospho-Thr308) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Akt1 (Phospho-Thr308).

Product Info Summary

SKU: EKC2635
Size: 1 kit, containing 2 plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

Akt1 (Phospho-Thr308) Colorimetric Cell-Based ELISA Kit

See all Akt1 products

SKU/Catalog Number

EKC2635

Size

1 kit, containing 2 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The Akt1 (Phospho-Thr308) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Akt1 (Phospho-Thr308) protein expression profile in cells. The kit can be used for measuring the relative amounts of Akt1 (Phospho-Thr308) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Akt1 (Phospho-Thr308).

Storage & Handling

4°C/6 Months

Cite This Product

Akt1 (Phospho-Thr308) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2635)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC2635 is reactive to AKT1 in Human, Mouse, Rat samples

Application Guarantee

EKC2635 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-AKT1 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-AKT1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For AKT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P31749

Gene ID

207

Gene Name

AKT1

Full Name

RAC-alpha serine/threonine-protein kinase

Weight

55.686kDa

Superfamily

protein kinase superfamily

Alternative Names

AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 AKT1 AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA AKT serine/threonine kinase 1 RAC-alpha serine/threonine-protein kinase|AKT1m|PKB alpha|RAC-PK-alpha|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha|serine-threonine protein kinase|v-akt murine thymoma viral oncogene homolog 1|v-akt murine thymoma viral oncogene-like protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on AKT1, check out the AKT1 Infographic

AKT1 infographic

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In this infographic you will see the following information for AKT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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