|1. Normal rabbit serum blocking reagent: 12 ml, for the block of tissue sections. |
|2. Biotinylated Secondary Antibody (Rabbit Anti-human IgG): 12ml (10ug/ml). Affinity purified antibody, labeled with “long-arm” biotin (Biotinamidohexanoic acid N-hydroxysuccinimide ester, CAS# 72040-63-2). |
|3. SABC-AP (Alkaline Phosphatase conjugated streptavidin): 12ml (20ug/ml). Manufactured by Boster’s proprietary method, the complex is very stable and offers superior amplification of the antigen signals. |
|4. BCIP/NBT Chromogenic agent (20x): 1ml. |
|5. Water soluble sealing reagent: 12 ml. |
|6. Neutral nuclear fast red: 12 ml.
Washing buffer Preparation: Disolve AR0030-E to 1000ml distilled water and adjust pH to 7.2~7.6. Finally, adjust the total volume to 1L.
*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.
See Prices For Extra Components