|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-14-3-3 Sigma Antibody|
|Description||Rabbit IgG polyclonal antibody for 14-3-3 protein sigma(SFN) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-14-3-3 Sigma Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1797)|
|Replacement Item||This antibody may replace the following items: sc-100638|sc-1019|sc-109424|sc-109425|sc-166473|sc-166726|sc-7681|sc-7683|sc-98414 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma(140-156aa KKRIIDSARSAYQEAMD), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||14-3-3 protein sigma|
|Molecular Weight||27774 MW|
|Protein Function||Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. .|
|Tissue Specificity||Present mainly in tissues enriched in stratified squamous keratinizing epithelium.|
|Subcellular Localization||Cytoplasm. Nucleus . Secreted. May be secreted by a non-classical secretory pathway.|
|Alternative Names||14-3-3 protein sigma;Epithelial cell marker protein 1;Stratifin;SFN;HME1;|
|Research Areas|||neuroscience|neurology process|neural signal transduction| signal transduction|adapters|cytoplasmic| neuroscience|cell type marker|neuron marker|synapse marker| stem cells|signaling pathways|wnt|14-3-3||
Background for 14-3-3 protein sigma
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-14-3-3 Sigma Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-14-3-3 Sigma Antibody Images
Click the images to enlarge.
All lanes: Anti 14-3-3 sigma (PA1797) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: SE620 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
IHC(P): Human Oesophagus Squama Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,