Anti-53BP1/TP53BP1 Antibody

53BP1 antibody

Boster Bio Anti-53BP1/TP53BP1 Antibody catalog # PA1976. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1976
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, ICC, WB

Product Name

Anti-53BP1/TP53BP1 Antibody

View all 53BP1 Antibodies

SKU/Catalog Number

PA1976

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-53BP1/TP53BP1 Antibody catalog # PA1976. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-53BP1/TP53BP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1976)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1, identical to the related mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1976 is reactive to TP53BP1 in Human, Mouse, Rat

Applications

PA1976 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

450 kDa

Calculated molecular weight

213.574kDa

Background of 53BP1

TP53BP1 (Tumor Protein p53-Binding Protein 1), also called 53BP1, is a protein that in humans is encoded by the TP53BP1 gene. Iwabuchi et al. (1998) mapped the TP53BP1 gene to 15q15-q21 by FISH. Iwabuchi et al. (1994) showed that TP53BP1 binds to the conformationally sensitive central domain of wildtype p53 but not to mutant p53 in vitro. Immunoblot analysis by Iwabuchi et al. (1998) showed that expression of TP53BP1 or TP53BP2 enhances the transactivation function of p53 and induces the expression of p21 (CDKN1A). Wang et al. (2002) used small interfering RNA directed against TP53BP1 in mammalian cells to demonstrate that TP53BP1 is a key transducer of the DNA damage checkpoint signal. TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For TP53BP1 (Source: Uniprot.org, NCBI)

Gene Name

TP53BP1

Full Name

TP53-binding protein 1

Weight

213.574kDa

Alternative Names

53BP1; FLJ41424; MGC138366; p202; p53-binding protein 1; p53bp1; TP53BP1; tumor protein 53-binding protein, 1; tumor protein p53 binding protein 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1 TP53BP1 53BP1, TDRD30, p202, p53BP1 tumor protein p53 binding protein 1 TP53-binding protein 1|p53-binding protein 1|tumor protein 53-binding protein, 1|tumor suppressor p53-binding protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TP53BP1, check out the TP53BP1 Infographic

TP53BP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TP53BP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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17 Customer Q&As for Anti-53BP1/TP53BP1 Antibody

Question

We need to test anti-53BP1/TP53BP1 antibody PA1976 on human cervix for research purposes, then I may be interested in using anti-53BP1/TP53BP1 antibody PA1976 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

The products we sell, including anti-53BP1/TP53BP1 antibody PA1976, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-10

Question

Do you have a BSA free version of anti-53BP1/TP53BP1 antibody PA1976 available?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-53BP1/TP53BP1 antibody PA1976 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-17

Question

We have tried in the past anti-53BP1/TP53BP1 antibody for IHC on cervix carcinoma erythroleukemia in the past. I am using rat, and We want to use the antibody for WB next. I am interested in examining cervix carcinoma erythroleukemia as well as embryonic kidney in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

I looked at the website and datasheets of our anti-53BP1/TP53BP1 antibody and it appears that PA1976 has been validated on rat in both IHC and WB. Thus PA1976 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-25

Question

Can you help my question with product PA1976, anti-53BP1/TP53BP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

L. Parker

Verified customer

Asked: 2019-06-10

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1976 anti-53BP1/TP53BP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-10

Question

Will PA1976 anti-53BP1/TP53BP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

As indicated on the product datasheet, PA1976 anti-53BP1/TP53BP1 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-31

Question

I was wanting to use your anti-53BP1/TP53BP1 antibody for ICC for human cervix on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix identification?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

You can see on the product datasheet, PA1976 anti-53BP1/TP53BP1 antibody has been validated for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-16

Question

I see that the anti-53BP1/TP53BP1 antibody PA1976 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-02-22

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-22

Question

Does anti-53BP1/TP53BP1 antibody PA1976 work on bovine WB with skeletal muscle?

Verified Customer

Verified customer

Asked: 2019-02-06

Answer

Our lab technicians have not validated anti-53BP1/TP53BP1 antibody PA1976 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-53BP1/TP53BP1 antibody PA1976 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine skeletal muscle in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-06

Question

See below the WB image, lot number and protocol we used for cervix using anti-53BP1/TP53BP1 antibody PA1976. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-12-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-25

Question

I would like using your anti-53BP1/TP53BP1 antibody for double-strand break repair via nonhomologous end joining studies. Has this antibody been tested with western blotting on hepa cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-04-26

Answer

Thank you for your inquiry. This PA1976 anti-53BP1/TP53BP1 antibody is tested on 293t cell lysate, hepa cell lysate, intestinal cancer tissue, rat brain tissue, mouse brain. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-04-26

Question

We are currently using anti-53BP1/TP53BP1 antibody PA1976 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

B. Jones

Verified customer

Asked: 2018-04-04

Answer

The anti-53BP1/TP53BP1 antibody (PA1976) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-04-04

Question

Is this PA1976 anti-53BP1/TP53BP1 antibody reactive to the isotypes of TP53BP1?

Verified Customer

Verified customer

Asked: 2017-10-18

Answer

The immunogen of PA1976 anti-53BP1/TP53BP1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-18

Question

We have seen staining in human myeloid leukemia cell. Do you have any suggestions? Is anti-53BP1/TP53BP1 antibody supposed to stain myeloid leukemia cell positively?

Verified Customer

Verified customer

Asked: 2017-09-01

Answer

From what I have seen in literature myeloid leukemia cell does express TP53BP1. From what I have seen in Uniprot.org, TP53BP1 is expressed in lung, skeletal muscle, myeloid leukemia cell, cervix, cerebellum, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have TP53BP1 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 15489334
Cervix, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 9748285

Boster Scientific Support

Answered: 2017-09-01

Question

We were satisfied with the WB result of your anti-53BP1/TP53BP1 antibody. However we have seen positive staining in lung nucleus using this antibody. Is that expected? Could you tell me where is TP53BP1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-08-29

Answer

Based on literature, lung does express TP53BP1. Generally TP53BP1 expresses in nucleus. Regarding which tissues have TP53BP1 expression, here are a few articles citing expression in various tissues:
Cerebellum, Pubmed ID: 15489334
Cervix, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 9748285

Boster Scientific Support

Answered: 2017-08-29

Question

Will anti-53BP1/TP53BP1 antibody PA1976 work for ICC with cervix?

A. Mitchell

Verified customer

Asked: 2016-08-10

Answer

According to the expression profile of cervix, TP53BP1 is highly expressed in cervix. So, it is likely that anti-53BP1/TP53BP1 antibody PA1976 will work for ICC with cervix.

Boster Scientific Support

Answered: 2016-08-10

Question

Is a blocking peptide available for product anti-53BP1/TP53BP1 antibody (PA1976)?

C. Collins

Verified customer

Asked: 2015-10-06

Answer

We do provide the blocking peptide for product anti-53BP1/TP53BP1 antibody (PA1976). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-10-06

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix using anti-53BP1/TP53BP1 antibody PA1976. Let me know if you need anything else.

B. Miller

Verified customer

Asked: 2015-06-10

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-06-10

Order DetailsPrice
PA1976

100μg

$370
PA1976-10ug

10μg sample (liquid)

$99
PA1976-Biotin

100 μg Biotin conjugated

$570
PA1976-Cy3

100 μg Cy3 conjugated

$570
PA1976-Dylight488

100 μg Dylight488 conjugated

$570
PA1976-Dylight550

100 μg Dylight550 conjugated

$570
PA1976-Dylight594

100 μg Dylight594 conjugated

$570
PA1976-FITC

100 μg FITC conjugated

$570
PA1976-HRP

100 μg HRP conjugated

$570
PA1976-APC

100 μg APC conjugated

$670
PA1976-PE

100 μg PE conjugated

$670
PA1976-carrier-free

Carrier Free

$370

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