|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-53BP1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Tumor suppressor p53-binding protein 1(TP53BP1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-53BP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1976)|
|Replacement Item||This antibody may replace the following items: sc-10911|sc-10914|sc-10915|sc-109897|sc-22760 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1(1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Tumor suppressor p53-binding protein 1|
|Molecular Weight||213574 MW|
|Protein Function||Plays a key role in the response to DNA damage. May have a role in checkpoint signaling during mitosis. Enhances TP53- mediated transcriptional activation. .|
|Sequence Similarities||Contains 2 BRCT domains.|
|Subcellular Localization||Nucleus. Chromosome, centromere, kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. H4K20me2 is required for efficient localization to double strand breaks and removal of proteins that have a high affinity for H4K20me2 such as L3MBTL1 and KDM4A is needed.|
|Alternative Names||Tumor suppressor p53-binding protein 1;53BP1;p53-binding protein 1;p53BP1;TP53BP1;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|dna damage & repair|dna damage response|p53| epigenetics and nuclear signaling|brct domain proteins| cancer|oncoproteins/suppressors|tumor suppressors|p53 pathway||
Background for Tumor suppressor p53-binding protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-53BP1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-53BP1 Antibody Images
Click the images to enlarge.
Lane 1: 293T Cell Lysate
Lane 2: U20S Cell Lysate
Lane 3: HEPA Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Rat Brain Tissue
IHC(P): Mouse Brain Tissue
ICC: A549 Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,