Anti-5HT1A Receptor/HTR1A Antibody

5-HT1A antibody

Boster Bio Anti-5HT1A Receptor/HTR1A Antibody catalog # PA1647. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: PA1647
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-5HT1A Receptor/HTR1A Antibody

View all 5-HT1A Antibodies

SKU/Catalog Number

PA1647

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-5HT1A Receptor/HTR1A Antibody catalog # PA1647. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-5HT1A Receptor/HTR1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1647)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT1A Receptor, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1647 is reactive to HTR1A in Human, Rat

Applications

PA1647 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

46 kDa

Calculated molecular weight

46.107kDa

Background of 5-HT1A

HTR1A (5-HYDROXYTRYPTAMINE RECEPTOR 1A), also called SEROTONIN 5-HT-1A RECEPTOR or BETA-2-ADRENERGIC RECEPTOR-LIKE PROTEIN G-21, is a subtype of 5-HT receptor that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. HTR1A denotes the human gene encoding for the receptor.The HTR1A gene is located at 5q12.3. The decreases in 5-HT-1A receptor densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5-HT-1A receptors has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. 5-HT-1A receptors in the dorsal raphe nucleus are co-localized with neurokinin 1 (NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For HTR1A (Source: Uniprot.org, NCBI)

Gene Name

HTR1A

Full Name

5-hydroxytryptamine receptor 1A

Weight

46.107kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

5-HT1a receptor; 5HT1A; 5-HT1A; 5-HT-1A; 5-HT1A5-hydroxytryptamine receptor 1A; 5-hydroxytryptamine (serotonin) receptor 1A; ADRB2RL1; ADRB2RL15HT1a; ADRBRL1; G protein coupled receptor; G-21; guanine nucleotide-binding regulatory protein-coupled receptor; HTR1A; PFMCD; Serotonin receptor 1A HTR1A 5-HT-1A, 5-HT1A, 5HT1a, ADRB2RL1, ADRBRL1, G-21, PFMCD 5-hydroxytryptamine receptor 1A 5-hydroxytryptamine receptor 1A|5-HT1a receptor|5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled|guanine nucleotide-binding regulatory protein-coupled receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HTR1A, check out the HTR1A Infographic

HTR1A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HTR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-5HT1A Receptor/HTR1A Antibody

Question

Will anti-5HT1A Receptor/HTR1A antibody PA1647 work for WB with brain?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

According to the expression profile of brain, HTR1A is highly expressed in brain. So, it is likely that anti-5HT1A Receptor/HTR1A antibody PA1647 will work for WB with brain.

Boster Scientific Support

Answered: 2020-03-26

Question

Do you have a BSA free version of anti-5HT1A Receptor/HTR1A antibody PA1647 available?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-5HT1A Receptor/HTR1A antibody PA1647 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-13

Question

Will anti-5HT1A Receptor/HTR1A antibody PA1647 work on goat IHC-P with brain?

Verified Customer

Verified customer

Asked: 2019-12-17

Answer

Our lab technicians have not tested anti-5HT1A Receptor/HTR1A antibody PA1647 on goat. You can run a BLAST between goat and the immunogen sequence of anti-5HT1A Receptor/HTR1A antibody PA1647 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain in IHC-P, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-17

Question

We are interested in to test anti-5HT1A Receptor/HTR1A antibody PA1647 on human brain for research purposes, then I may be interested in using anti-5HT1A Receptor/HTR1A antibody PA1647 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

The products we sell, including anti-5HT1A Receptor/HTR1A antibody PA1647, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-19

Question

We are currently using anti-5HT1A Receptor/HTR1A antibody PA1647 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

J. Kulkarni

Verified customer

Asked: 2018-05-21

Answer

The anti-5HT1A Receptor/HTR1A antibody (PA1647) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-21

Question

Is this PA1647 anti-5HT1A Receptor/HTR1A antibody reactive to the isotypes of HTR1A?

Verified Customer

Verified customer

Asked: 2018-04-03

Answer

The immunogen of PA1647 anti-5HT1A Receptor/HTR1A antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT1A Receptor(404-422aa NKDFQNAFKKIIKCKFCRQ), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-04-03

Question

Does PA1647 anti-5HT1A Receptor/HTR1A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Moore

Verified customer

Asked: 2017-09-12

Answer

You can see on the product datasheet, PA1647 anti-5HT1A Receptor/HTR1A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-12

Order DetailsPrice
PA1647

100μg

$370
PA1647-10ug

10μg sample (liquid)

$99
PA1647-Biotin

100 μg Biotin conjugated

$570
PA1647-Cy3

100 μg Cy3 conjugated

$570
PA1647-Dylight488

100 μg Dylight488 conjugated

$570
PA1647-Dylight550

100 μg Dylight550 conjugated

$570
PA1647-Dylight594

100 μg Dylight594 conjugated

$570
PA1647-FITC

100 μg FITC conjugated

$570
PA1647-HRP

100 μg HRP conjugated

$570
PA1647-APC

100 μg APC conjugated

$670
PA1647-PE

100 μg PE conjugated

$670
PA1647-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1647
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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