Product Info Summary
SKU: | M01498 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody
View all alpha 2-Macroglobulin Antibodies
SKU/Catalog Number
M01498
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody catalog # M01498. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01498)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABAO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human A2M
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01498 is reactive to A2M in Human
Applications
M01498 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
163.291kDa
Background of alpha 2-Macroglobulin
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of A2M expression in human plasma lysate.
Protein Target Info & Infographic
Gene/Protein Information For A2M (Source: Uniprot.org, NCBI)
Gene Name
A2M
Full Name
Alpha-2-macroglobulin
Weight
163.291kDa
Superfamily
protease inhibitor I39 (alpha-2-macroglobulin) family
Alternative Names
A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7 A2M A2MD, CPAMD5, FWP007, S863-7 alpha-2-macroglobulin alpha-2-macroglobulin|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5|alpha-2-M
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on A2M, check out the A2M Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for A2M: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody (M01498)
Hello CJ!
No publications found for M01498
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal Antibody
Question
May I know what is the concentration of the antibody in 100 µl? Have you tested it in ELISA?
Verified customer
Asked: 2022-09-07
Answer
The concentration of M01498 is 0.25mg/ml. We screen the antibody in ELISA with the antigen.
Boster Scientific Support
Answered: 2022-09-08
Question
Will anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal antibody M01498 work on horse WB with skin?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
Our lab technicians have not tested anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal antibody M01498 on horse. You can run a BLAST between horse and the immunogen sequence of anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal antibody M01498 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal antibody M01498 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
K. Jackson
Verified customer
Asked: 2017-03-06
Answer
The anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal antibody (M01498) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-06
Question
Is there a BSA free version of anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal antibody M01498 available?
C. Parker
Verified customer
Asked: 2014-02-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-A2M/Alpha 2 Macroglobulin Rabbit Monoclonal antibody M01498 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-02-13