Product Info Summary
SKU: | PB9123 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-AAMP CCL18 Antibody Picoband™
View all CCL18/PARC Antibodies
SKU/Catalog Number
PB9123
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AAMP CCL18 Antibody Picoband™ catalog # PB9123. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AAMP CCL18 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9123)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AAMP recombinant protein (Position: E235-R434).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9123 is reactive to CCL18 in Human
Applications
PB9123 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
47 kDa
Calculated molecular weight
9.849kDa
Background of CCL18/PARC
AAMP, also known as Angio-associated, migratory cell protein, is a protein which in humans is encoded by the AAMP gene. It is mapped to 2q35. The gene product of AAMP is an immunoglobulin-type protein, which is found to be expressed strongly in endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics. It has been demonstrated that an AAMP peptide containing the putative heparan sulfate-binding domain binds to heparin and mediates heparin-sensitive cell adhesion. AAMP plays a role in angiogenesis and cell migration. In smooth muscle cell migration, it may act through the RhoA pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AAMP using anti-AAMP antibody (PB9123).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human AAMP protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (Catalog # PB9123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AAMP at approximately 39 kDa. The expected band size for AAMP is at 39 kDa.
Click image to see more details
Figure 2. Western blot analysis of AAMP using anti-AAMP antibody (PB9123).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (Catalog # PB9123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AAMP at approximately 47 kDa. The expected band size for AAMP is at 10 kDa.
Click image to see more details
Figure 3. IHC analysis of AAMP using anti-AAMP antibody (PB9123).
AAMP was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AAMP Antibody (PB9123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of AAMP using anti-AAMP antibody (PB9123).
AAMP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AAMP Antibody (PB9123) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of K562 cells using anti-AAMP antibody (PB9123).
Overlay histogram showing K562 cells stained with PB9123 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AAMP Antibody (PB9123,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CCL18 (Source: Uniprot.org, NCBI)
Gene Name
CCL18
Full Name
C-C motif chemokine 18
Weight
9.849kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated CCL18 AMAC-1, AMAC1, CKb7, DC-CK1, DCCK1, MIP-4, PARC, SCYA18 C-C motif chemokine ligand 18 C-C motif chemokine 18|CC chemokine PARC|CC chemokine ligand 18|alternative macrophage activation-associated CC chemokine 1|chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)|chemokine (C-C), dendritic|dendritic cell chemokine 1|macrophage inflammatory protein 4|pulmonary and activation-regulated chemokine|small inducible cytokine A18|small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCL18, check out the CCL18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCL18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AAMP CCL18 Antibody Picoband™ (PB9123)
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-AAMP CCL18 Antibody Picoband™
Question
Will anti-AAMP antibody PB9123 work for IHC with ovary skin?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
According to the expression profile of ovary skin, AAMP is highly expressed in ovary skin. So, it is likely that anti-AAMP antibody PB9123 will work for IHC with ovary skin.
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-AAMP antibody PB9123 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-AAMP antibody (PB9123) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
Question
Is there a BSA free version of anti-AAMP antibody PB9123 available?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AAMP antibody PB9123 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-19
Question
Is a blocking peptide available for product anti-AAMP antibody (PB9123)?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
We do provide the blocking peptide for product anti-AAMP antibody (PB9123). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary skin using anti-AAMP antibody PB9123. Let me know if you need anything else.
J. Li
Verified customer
Asked: 2017-12-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-18
Question
My question regards to test anti-AAMP antibody PB9123 on human ovary skin for research purposes, then I may be interested in using anti-AAMP antibody PB9123 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Carter
Verified customer
Asked: 2017-08-11
Answer
The products we sell, including anti-AAMP antibody PB9123, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-11