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SKU:PA1843
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-ABCA1 Antibody
Description Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family A member 1(ABCA1) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-ABCA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1843)
Replacement Item This antibody may replace the following items: sc-20794|sc-53482|sc-5490|sc-5491|sc-5492|sc-58219|sc-81779 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human ABCA1(2219-2236aa NFAKDQSDDDHLKDLSLH), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ABCA1
Protein Name ATP-binding cassette sub-family A member 1
Molecular Weight 254302 MW
Protein Function cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
Tissue Specificity Widely expressed, but most abundant in macrophages.
Sequence Similarities Belongs to the ABC transporter superfamily. ABCA family.
Subcellular Localization Membrane ; Multi-pass membrane protein .
Uniprot ID O95477
Alternative Names ATP-binding cassette sub-family A member 1;ATP-binding cassette transporter 1;ABC-1;ATP-binding cassette 1;Cholesterol efflux regulatory protein;ABCA1;ABC1, CERP;
Research Areas |neuroscience|cell type marker|glia marker|astrocyte marker| cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism| signal transduction|atherosclerosis|lipoprotein metabolism| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|heart disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for ATP-binding cassette sub-family A member 1

ABCA1(ATP-binding cassette, sub-family A(ABC1), member 1), also known as ABC1, the cholesterol efflux regulatory protein(CERP), is a protein which in humans is encoded by the ABCA1 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette(ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes,contains 2,261 amino acids. Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, with highest expression in placenta, liver, lung, adrenal glands, and all fetal tissues examined, and lowest expression in kidney, pancreas, pituitary, mammary gland, and bone marrow. This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterolefflux  pump in the cellular lipid removal pathway. Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, Szakacs et al. found specific, Mg(2+)-dependent ATP binding and low basal ATPase activity. Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1. Szakacs et al. speculated that ABCA1 may be a regulatory protein or may require other protein partners for full activation.

Anti-ABCA1 Antibody Images

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Anti-ABCA1 Antibody
Anti-ABCA1 antibody, PA1843, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: HT1080 Cell Lysate
Lane 3: HELA Cell Lysate
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Publications

Chen M. Int J Biomed Sci. 2013 Sep;9(3):153-61. Effects Of Chinese Herbal Compound "Xuemai Ning"On Rabbit Atherosclerosis Model And Expression Of Abca1.
Yan N, Ding T, Dong J, Li Y, Wu M. Lipids Health Dis. 2011 Mar 21;10:46. Doi: 10.1186/1476-511X-10-46. Sphingomyelin Synthase Overexpression Increases Cholesterol Accumulation And Decreases Cholesterol Secretion In Liver Cells.
Zhong Jk, Guo Zg, Li C, Wang Zk, Lai Wy, Tu Y. Lipids Health Dis. 2011 Nov 12;10:210. Doi: 10.1186/1476-511X-10-210. Probucol Alleviates Atherosclerosis And Improves High Density Lipoprotein Function.
Li C, Tu Y, Liu Tr, Guo Zg, Xie D, Zhong Jk, Fan Yz, Lai Wy. Int J Mol Med. 2015 Mar;35(3):715-23. Doi: 10.3892/Ijmm.2015.2072. Epub 2015 Jan 19. Rosiglitazone Attenuates Atherosclerosis And Increases High-Density Lipoprotein Function In Atheroscl...
Shang W, Yu X, Wang H, Chen T, Fang Y, Yang X, Zhou P, Nie F, Zhou Q, Zhou J. Mol Med Rep. 2015 Jan;11(1):503-8. Doi: 10.3892/Mmr.2014.2731. Epub 2014 Oct 21. Fibroblast Growth Factor 21 Enhances Cholesterol Efflux In Thp-1 Macrophage-Derived Foam...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to abca1 antibody, membrane bound antibody