Product Info Summary
SKU: | PB9275 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-ABCB4 Antibody Picoband™
View all MDR3/ABCB4 Antibodies
SKU/Catalog Number
PB9275
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABCB4 Antibody Picoband™ catalog # PB9275. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCB4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9275)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ABCB4 recombinant protein (Position: A601-A720). Human ABCB4 shares 79% amino acid (aa) sequence identity with both mouse and rat ABCB4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9275 is reactive to ABCB4 in Human, Mouse, Rat
Applications
PB9275 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
150 kDa
Calculated molecular weight
141.523kDa
Background of MDR3/ABCB4
Adenosine triphosphate-binding cassette subfamily B, member 4 (ABCB4), also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The ABCB4 gene contains 28 exons and 27 of these contain coding sequences for the two homologous halves of the protein that correlate with functional domains. ABCB4 gene mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABCB4 using anti-ABCB4 antibody (PB9275).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat RH35 whole cell lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody (Catalog # PB9275) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 150 kDa. The expected band size for ABCB4 is at 142 kDa.
Click image to see more details
Figure 2. IHC analysis of ABCB4 using anti-ABCB4 antibody (PB9275).
ABCB4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB4 Antibody (PB9275) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. Flow Cytometry analysis of HepG2 cells using anti-ABCB4 antibody (PB9275).
Overlay histogram showing HepG2 cells stained with PB9275 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB4 Antibody (PB9275, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ABCB4 (Source: Uniprot.org, NCBI)
Gene Name
ABCB4
Full Name
Phosphatidylcholine translocator ABCB4
Weight
141.523kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABC21; ABCB4; ATP-binding cassette sub-family B member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4; EC 3.6.3; EC 3.6.3.44; GBD1; MDR2; MDR3; MDR3MDR2/3; multidrug resistance protein 3; multiple drug resistance 3; P glycoprotein 3/multiple drug resistance 3; PFIC-3; P-glycoprotein 3; P-glycoprotein-3/multiple drug resistance-3; PGY3; PGY3GBD1 ABCB4 ABC21, GBD1, ICP3, MDR2, MDR2/3, MDR3, PFIC-3, PGY3 ATP binding cassette subfamily B member 4 phosphatidylcholine translocator ABCB4|ATP-binding cassette sub-family B member 4|ATP-binding cassette, sub-family B (MDR/TAP), member 4|P-glycoprotein 3|P-glycoprotein-3/multiple drug resistance-3|multidrug resistance protein 3|multiple drug resistance 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCB4, check out the ABCB4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCB4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCB4 Antibody Picoband™ (PB9275)
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Customer Q&As
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7 Customer Q&As for Anti-ABCB4 Antibody Picoband™
Question
Would anti-ABCB4 antibody PB9275 work for ICC with liver?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
According to the expression profile of liver, ABCB4 is highly expressed in liver. So, it is likely that anti-ABCB4 antibody PB9275 will work for ICC with liver.
Boster Scientific Support
Answered: 2020-03-16
Question
I have a question about product PB9275, anti-ABCB4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9275 anti-ABCB4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-16
Question
Would anti-ABCB4 antibody PB9275 work on zebrafish WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
Our lab technicians have not tested anti-ABCB4 antibody PB9275 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ABCB4 antibody PB9275 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish right lobe of liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-01
Question
I was wanting to use your anti-ABCB4 antibody for ICC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
It shows on the product datasheet, PB9275 anti-ABCB4 antibody has been validated for Flow Cytometry, IF, IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-25
Question
Is a blocking peptide available for product anti-ABCB4 antibody (PB9275)?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
We do provide the blocking peptide for product anti-ABCB4 antibody (PB9275). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-26
Question
We are currently using anti-ABCB4 antibody PB9275 for mouse tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
The anti-ABCB4 antibody (PB9275) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ABCB4 antibody PB9275. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-29