Anti-ABCB5 Antibody

Abcb5 antibody

Boster Bio Anti-ABCB5 Antibody (Catalog # A02979). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A02979
Size: 100ug
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-ABCB5 Antibody

View all Abcb5 Antibodies

SKU/Catalog Number

A02979

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ABCB5 Antibody (Catalog # A02979). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store Anti-ABCB5 at -20°C prior to opening. Aliquot antibody and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-ABCB5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02979)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02979 is reactive to Abcb5 in Human

Applications

A02979 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

137.397kDa

Background of Abcb5

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-ABCB5 Antibody is a ATP-Binding Cassette protein. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5. ABCB5 antibody is ideal for Cancer and Signal Transduction research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:200
WB: 1:20,000

Validation Images & Assay Conditions

Gene/Protein Information For Abcb5 (Source: Uniprot.org, NCBI)

Gene Name

Abcb5

Full Name

ATP-binding cassette sub-family B member 5

Weight

137.397kDa

Superfamily

ABC transporter superfamily

Alternative Names

ABCB5 P-gp; ABCB5; ABCB5alpha; ABCB5beta; ATP-binding cassette protein; ATP-binding cassette sub-family B member 5; ATP-binding cassette, sub-family B (MDR/TAP), member 5; EC 3.6.3; EC 3.6.3.44; EST422562; P-glycoprotein ABCB5 Abcb5|9230106F14Rik|ATP-binding cassette, sub-family B (MDR/TAP), member 5|ATP-binding cassette sub-family B member 5|ABCB5 P-gp|P-glycoprotein ABCB5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Abcb5, check out the Abcb5 Infographic

Abcb5 infographic

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In this infographic, you will see the following information for Abcb5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-ABCB5 Antibody

Question

Do you have a BSA free version of anti-ABCB5 antibody A02979 available?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ABCB5 antibody A02979 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-25

Question

Is a blocking peptide available for product anti-ABCB5 antibody (A02979)?

M. Wu

Verified customer

Asked: 2018-09-27

Answer

We do provide the blocking peptide for product anti-ABCB5 antibody (A02979). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-09-27

Question

Please see the WB image, lot number and protocol we used for melanoma using anti-ABCB5 antibody A02979. Please let me know if you require anything else.

M. Evans

Verified customer

Asked: 2017-02-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-02-08

Question

Is this A02979 anti-ABCB5 antibody reactive to the isotypes of ABCB5?

R. Johnson

Verified customer

Asked: 2013-01-21

Answer

The immunogen of A02979 anti-ABCB5 antibody is ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-01-21

Order DetailsPrice
A02979

100 μg

$599

Free Secondary Antibody

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A02979
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$599.00

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