Data & Images
|Product Name||Anti-ABCG1 Antibody|
|Description||Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family G member 1(ABCG1) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-ABCG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1868)|
|Replacement Item||This antibody may replace the following items: sc-21808|sc-20795|sc-11150 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ABCG1(627-647aa ETCHFQKSEAILRELDVENAK), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||ATP-binding cassette sub-family G member 1|
|Molecular Weight||75592 MW|
|Protein Function||Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.|
|Tissue Specificity||Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease. .|
|Sequence Similarities||Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.|
|Subcellular Localization||Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.|
|Alternative Names||ATP-binding cassette sub-family G member 1;ATP-binding cassette transporter 8;White protein homolog;ABCG1;ABC8, WHT1;|
|Research Areas|||neuroscience|cell type marker|glia marker|astrocyte marker| cardiovascular|lipids / lipoproteins|lipid metabolism|cholesterol metabolism|lipoproteins/apolipoproteins| signal transduction|atherosclerosis|lipoprotein metabolism|lipid transport| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|heart disease||
Background for ATP-binding cassette sub-family G member 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ABCG1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ABCG1 Antibody Images
Click the images to enlarge.
All lanes: Anti ABCG1 (PA1868) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,