Overview
| Product Name |
Anti-ACE2 Antibody
See more ACE-2 products |
| Catalog# |
A00756 |
| Pack Size |
0.1 mg |
| Storage & Handling |
At -20°C for one year. At 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. |
| Description |
Anti-ACE2 Antibody validated for ELISA, WB, IHC, IF. Reactive to Human, Mouse. |
| Cite This Product |
Anti-ACE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00756)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse |
| Applications |
ELISA, IF, IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
ACE2 antibody was raised against a synthetic peptide corresponding to amino acids near the center of human ACE2.
The immunogen is located within amino acids 180 - 230 of ACE2. |
Gene/Protein Basic Information For ACE2 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9BYF1 |
|---|
| NCBI Gene Id | 59272 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | ACE2 |
|---|
| Protein Name | Angiotensin-converting enzyme 2 |
|---|
| Superfamily | peptidase M2 family |
|---|
| Alternative Names | ACE-2|ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15 |
|---|
| Molecular Weight | 92463 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ACE2, check out the ACE2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ACE2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the ACE2 infographic
Our Boster Quality Guarantee for Anti-ACE2 Antibody covers its use in the following applications.
ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 μg/mL. A 90 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 6. Immunofluorescent staining data of ACE2 using Anti-ACE2 Antibody (A00756).
Immunofluorescence Validation of ACE2 in Human Kidney Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with A00756 at 20 μg/mL
Figure 5. Immunohistochemistry validation of ACE2 using Anti-ACE2 Antibody (A00756).
Immunohistochemistry Validation of ACE2 in Human Kidney Tissue
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (A00756) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
For more protocol information of IHC
Figure 1. Western blotting validation for Anti-ACE2 Antibody A00756
15 μg of lysates per lane.
Antibodies: ACE2
Figure 2. Western blotting validation for Anti-ACE2 Antibody A00756
15 μg of lysates per lane.
Antibodies: ACE2
Figure 3. Western blotting validation for Anti-ACE2 Antibody A00756
15 μg of lysates per lane.
Antibodies: ACE2
Figure 4. Western blotting validation for Anti-ACE2 Antibody A00756
15 μg of lysates per lane.
Antibodies: ACE2
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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