SKU MA1000
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number AC-40
Host Mouse
Ig Isotype Mouse IgG2a
Applications IHC, WB

Overview

Product Name Anti-Actin Antibody (Monoclonal, AC-40)
SKU/Catalog Number MA1000
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Actin detection. Tested with WB, IHC-F in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Actin Antibody (Monoclonal, AC-40) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1000)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Form Lyophilized
Immunogen Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide(MAP) backbone.
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Frozen Section), 4μg/ml, Human, chicken, mouse, rat, -
Western blot, 2μg/ml, Human, chicken, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Images And Assay Conditions

/antibody/ma1000 1 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Mouse Intestine Tissue

/antibody/ma1000 2_2 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Mouse Spleen Tissue

/antibody/ma1000 3 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Rat Intestine Tissue

/antibody/ma1000 4 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Rat Spleen Tissue

/antibody/ma1000 5 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Human Placenta Tissue

/M/A/MA1000 6.jpg

Figure 6. Western blot analysis of Actin using anti-Actin antibody (MA1000).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse NIH3T3 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Actin antigen affinity purified monoclonal antibody (Catalog # MA1000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Actin at approximately 43KD. The expected band size for Actin is at 43KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P68133
Gene Name Actin
Protein Name Actin, alpha skeletal muscle
Alternative Names Actin, alpha skeletal muscle;Alpha-actin-1;ACTA1;ACTA;
Subcellular Localization Cytoplasm, cytoskeleton.
Molecular Weight 42051 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Research Areas Actin, Actin Assembly, Actin Binding Proteins, Actin Etc, Apoptosis, Cancer, Cell Death, Cytoskeleton, Cytoskeleton / Ecm, Invasion/Microenvironment, Microfilaments, Receptors, Signal Transduction, Subcellular Markers, Tags & Cell Markers

*You can search these to find other products in these research areas.
Background Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

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Order Product (MA1000)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships next business day.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is gamma-actin antibody