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SKU:MA1000
Clonality:Monoclonal
Clone Number:AC-40
Application:IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Actin Antibody (Monoclonal, AC-40)
Description Mouse IgG monoclonal antibody for Actin detection. Tested with WB, IHC-F in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Actin Antibody (Monoclonal, AC-40) AC-40 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1000)
Replacement Item This antibody may replace the following items: sc-8432|sc-376421|sc-10731|sc-7210|sc-81760|sc-56459|sc-58679|sc-70319|sc-58673|sc-1616|sc-1615|sc-1616-R|sc-1615-R|sc-1615-X from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2a
Validated Species Chicken, Human, Mouse, Rat
Predicted Species Chicken

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide(MAP) backbone.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number AC-40
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ACTA1
Protein Name Actin, alpha skeletal muscle|Actin, alpha cardiac muscle 1|Actin, aortic smooth muscle|Actin, cytoplasmic 1|Actin, cytoplasmic 2|Actin, gamma-enteric smooth muscle
Molecular Weight 42051 MW
Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Localization Cytoplasm, cytoskeleton.
Uniprot ID P68133
Alternative Names Actin, alpha skeletal muscle;Alpha-actin-1;ACTA1;ACTA;
Research Areas Actin|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Actin, alpha skeletal muscle|Actin, alpha cardiac muscle 1|Actin, aortic smooth muscle|Actin, cytoplasmic 1|Actin, cytoplasmic 2|Actin, gamma-enteric smooth muscle

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Anti-Actin Antibody (Monoclonal, AC-40) Images

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Anti-Actin Antibody (Monoclonal, AC-40)
Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Mouse Intestine Tissue
Anti-Actin Antibody (Monoclonal, AC-40)
Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Mouse Spleen Tissue
Anti-Actin Antibody (Monoclonal, AC-40)
Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Rat Intestine Tissue
Anti-Actin Antibody (Monoclonal, AC-40)
Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Rat Spleen Tissue
Anti-Actin Antibody (Monoclonal, AC-40)
Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Human Placenta Tissue
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Publications

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Wang Yl, Liu Lz, He Zh, Ding Kh, Xue F. Exp Ther Med. 2012 Jul;4(1):26-32. Epub 2012 Apr 17. Phenotypic Transformation And Migration Of Adventitial Cells Following Angioplasty.
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Li X, Zhao L, Chen Z, Lin Y, Yu P, Mao L. Anal Chem. 2012 Jun 19;84(12):5285-91. Doi: 10.1021/Ac300354Z. Epub 2012 May 25. Continuous Electrochemical Monitoring Of Extracellular Lactate Production From Neonatal Rat Cardiomyocytes Following Myocard...
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Du Y, Zhang S, Wang Z, Zhou W, Luan M, Yang X, Chen N. Oncol Rep. 2008 Sep;20(3):605-11. Induction Of Apoptosis And Cell Cycle Arrest By Ns398 In Oral Squamous Cell Carcinoma Cells Via Downregulation Of E2 Promoter-Binding Factor-1.
Tang Wx, Chen Xp, Zhao Yy, Wang Jh, Wang W, Chen Zw. Hybridoma (Larchmt). 2007 Oct;26(5):338-41. Preparation And Identification Of Monoclonal Antibodies Against Recombinant Human Thioredoxins.
Yu M, Ma M, Huang C, Yang H, Lai J, Yan S, Li L, Xiang M, Tan D. Cancer Invest. 2009 Dec;27(10):978-83. Doi: 10.3109/07357900902769723. Correlation Of Expression Of Human Arrest-Defective-1 (Hard1) Protein With Breast Cancer.
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Chen Y, Liu W, Li W, Gao C. Exp Mol Pathol. 2010 Apr;88(2):311-5. Doi: 10.1016/J.Yexmp.2009.12.002. Epub 2009 Dec 11. Autologous Bone Marrow Mesenchymal Cell Transplantation Improves Left Ventricular Function In A Rabbit Model Of Dilated Cardiomyo...
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is gamma-actin antibody