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SKU:PA1324
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Actin Antibody
Description Rabbit IgG polyclonal antibody for Actin, cytoplasmic 2(ACTG1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Actin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1324)
Replacement Item This antibody may replace the following items: sc-53015 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Actin(365-375aa SGPSIVHRKCF), identical to the related mouse and rat sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Liquid
Contents Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year, at 4˚C for one month. Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ACTG1
Protein Name Actin, cytoplasmic 2
Molecular Weight 41793 MW
Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Sequence Similarities Belongs to the actin family.
Subcellular Localization Cytoplasm, cytoskeleton.
Uniprot ID P63261
Alternative Names Actin, cytoplasmic 2;Gamma-actin;Actin, cytoplasmic 2, N-terminally processed;ACTG1;ACTG;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin| stem cells|mesenchymal stem cells|myogenesis| cardiovascular|heart|contractility|contractile proteins|actins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Actin, cytoplasmic 2

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Anti-Actin Antibody Images

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Anti-Actin Antibody
Anti-Actin antibody, PA1324, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Skeletal Muscle Tissue Lysate
Lane 5: MM231 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: HT1080 Cell Lysate
Lane 9: SW620 Cell Lysate
Anti-Actin Antibody
Anti-Actin antibody, PA1324, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Anti-Actin Antibody
Anti-Actin antibody, PA1324, IHC(P)
All lanes: Anti -Actin (PA1324) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: Mouse Spleen Tissue Lysate at 50ug
Lane 3: Mouse Thymus Tissue Lysate at 50ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
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Publications

Chen J, Chen C, Chen Z, Chen J, Li Q, Huang N. J Biomed Mater Res A. 2010 Nov;95(2):341-9. Doi: 10.1002/Jbm.A.32847. Collagen/Heparin Coating On Titanium Surface Improves The Biocompatibility Of Titanium Applied As A Blood-Contacting Biomaterial.
Chen F, Yang X, Pang D, Peng Z, Dong M, Liu X, Ouyang H, Ren L. Int J Biol Macromol. 2014 Jun;67:289-94. Doi: 10.1016/J.Ijbiomac.2014.03.043. Epub 2014 Mar 30. Expression, Purification And Antibody Preparation Using Different Constructs Of Pcv2 Ca...
Wang Yl, Liu Lz, He Zh, Ding Kh, Xue F. Exp Ther Med. 2012 Jul;4(1):26-32. Epub 2012 Apr 17. Phenotypic Transformation And Migration Of Adventitial Cells Following Angioplasty.
Li M, Shang Yx, Wei B, Yang Yg. J Inflamm (Lond). 2011 Jul 21;8(1):18. Doi: 10.1186/1476-9255-8-18. The Effect Of Substance P On Asthmatic Rat Airway Smooth Muscle Cell Proliferation, Migration, And Cytoplasmic Calcium Concentration In Vitro.
Li M, Shang Yx. Bmc Pulm Med. 2012 Dec 17;12:79. Doi: 10.1186/1471-2466-12-79. Inhaled Corticosteroids Inhibit Substance P Receptor Expression In Asthmatic Rat Airway Smooth Muscle Cells.
Yang J, Shi Qd, Song Tb, Feng Gf, Zang Wj, Zong Ch, Chang L. Peptides. 2013 Apr;42:105-11. Doi: 10.1016/J.Peptides.2013.01.007. Epub 2013 Jan 20. Vasoactive Intestinal Peptide Increases Vegf Expression To Promote Proliferation Of Brain Vascular En...
Sun Q, Zhang Y, Yang G, Chen X, Zhang Y, Cao G, Wang J, Sun Y, Zhang P, Fan M, Shao N, Yang X. Nucleic Acids Res. 2008 May;36(8):2690-9. Doi: 10.1093/Nar/Gkn032. Epub 2008 Mar 19. Transforming Growth Factor-Beta-Regulated Mir-24 Promotes Skeletal ...
Liu N, Huang H, Lin F, Chen A, Zhang Y, Chen R, Du H. Neuroscience. 2011 Oct 27;194:349-58. Doi: 10.1016/J.Neuroscience.2011.07.037. Epub 2011 Jul 27. Effects Of Treadmill Exercise On The Expression Of Netrin-1 And Its Receptors In Rat Brain After...
Lu Yh, Wang K, He R, Xi T. Cancer Biother Radiopharm. 2008 Dec;23(6):727-34. Doi: 10.1089/Cbr.2008.0503. Knockdown Of Survivin And Upregulation Of P53 Gene Expression By Small Interfering Rna Induces Apoptosis In Human Gastric Carcinoma Cell Line ...
Zhang Gl, Dai Dz, Xi T, Cong Xd, Zhang Y, Dai Y. Acta Pharmacol Sin. 2011 Feb;32(2):223-9. Doi: 10.1038/Aps.2010.177. Isoproterenol-Induced Fkbp12.6/12 Downregulation Is Modulated By Eta And Etb Receptors And Reversed By Argirhein, A Derivative Of...
Xie Y, Hao H, Kang A, Liang Y, Xie T, Sun S, Dai C, Zheng X, Xie L, Li J, Wang G. J Ethnopharmacol. 2010 Sep 15;131(2):290-9. Doi: 10.1016/J.Jep.2010.06.038. Epub 2010 Jun 30. Integral Pharmacokinetics Of Multiple Lignan Components In Normal, Ccl4...
Li X, Zhao L, Chen Z, Lin Y, Yu P, Mao L. Anal Chem. 2012 Jun 19;84(12):5285-91. Doi: 10.1021/Ac300354Z. Epub 2012 May 25. Continuous Electrochemical Monitoring Of Extracellular Lactate Production From Neonatal Rat Cardiomyocytes Following Myocard...
Luo Q, Jiang L, Chen G, Feng Y, Lv Q, Zhang C, Qu S, Zhu H, Zhou B, Xiao X. Free Radic Res. 2011 Nov;45(11-12):1355-65. Doi: 10.3109/10715762.2011.627330. Constitutive Heat Shock Protein 70 Interacts With ??-Enolase And Protects Cardiomyocytes Agai...
Du Y, Zhang S, Wang Z, Zhou W, Luan M, Yang X, Chen N. Oncol Rep. 2008 Sep;20(3):605-11. Induction Of Apoptosis And Cell Cycle Arrest By Ns398 In Oral Squamous Cell Carcinoma Cells Via Downregulation Of E2 Promoter-Binding Factor-1.
Tang Wx, Chen Xp, Zhao Yy, Wang Jh, Wang W, Chen Zw. Hybridoma (Larchmt). 2007 Oct;26(5):338-41. Preparation And Identification Of Monoclonal Antibodies Against Recombinant Human Thioredoxins.
Yu M, Ma M, Huang C, Yang H, Lai J, Yan S, Li L, Xiang M, Tan D. Cancer Invest. 2009 Dec;27(10):978-83. Doi: 10.3109/07357900902769723. Correlation Of Expression Of Human Arrest-Defective-1 (Hard1) Protein With Breast Cancer.
Xia L, Liang H, Huang Y, Ou-Yang Z, Qin Q. Virus Res. 2010 Jan;147(1):30-9. Doi: 10.1016/J.Virusres.2009.09.015. Epub 2009 Oct 1. Identification And Characterization Of Singapore Grouper Iridovirus (Sgiv) Orf162L, An Immediate-Early Gene Involved ...
Chen Y, Liu W, Li W, Gao C. Exp Mol Pathol. 2010 Apr;88(2):311-5. Doi: 10.1016/J.Yexmp.2009.12.002. Epub 2009 Dec 11. Autologous Bone Marrow Mesenchymal Cell Transplantation Improves Left Ventricular Function In A Rabbit Model Of Dilated Cardiomyo...
Zhiguang X, Wei C, Steven R, Wei D, Wei Z, Rong M, Zhanguo L, Lianfeng Z. Immunol Lett. 2010 Jul 8;131(2):159-65. Doi: 10.1016/J.Imlet.2010.04.005. Epub 2010 Apr 20. Over-Expression Of Il-33 Leads To Spontaneous Pulmonary Inflammation In Mil-33 Tr...
Sun Yh, Li Yq, Feng Sl, Li Bx, Pan Zw, Xu Cq, Li Tt, Yang Bf. Biochem Biophys Res Commun. 2010 Apr 16;394(4):955-61. Doi: 10.1016/J.Bbrc.2010.03.096. Epub 2010 Mar 20. Calcium-Sensing Receptor Activation Contributed To Apoptosis Stimulates Trpc6 C...
Lu X, Cheng C, Zhu S, Yang Y, Zheng L, Wang G, Shu X, Wu K, Liu K, Tong Q. Oncol Rep. 2010 Oct;24(4):981-7. Satb1 Is An Independent Prognostic Marker For Gastric Cancer In A Chinese Population.
Wang Yh, Wu Mw, Yang Ak, Zhang Wd, Sun J, Liu Tr, Chen Yf. Med Oncol. 2011 Dec;28 Suppl 1:S360-6. Doi: 10.1007/S12032-010-9737-3. Epub 2010 Nov 11. Cox-2 Gene Increases Tongue Cancer Cell Proliferation And Invasion Through Vegf-C Pathway.
Liao L, Zheng R, Wang C, Gao J, Ying Y, Ning Q, Luo X. Pediatr Res. 2011 Jun;69(6):497-503. Doi: 10.1203/Pdr.0B013E31821769Bd. The Influence Of Down-Regulation Of Suppressor Of Cellular Signaling Proteins By Rnai On Glucose Transport Of Intrauteri...
Feng Sl, Sun Mr, Li Tt, Yin X, Xu Cq, Sun Yh. Biochem Biophys Res Commun. 2011 Mar 11;406(2):278-84. Doi: 10.1016/J.Bbrc.2011.02.033. Epub 2011 Feb 18. Activation Of Calcium-Sensing Receptor Increases Trpc3 Expression In Rat Cardiomyocytes.
Yang L, Ye D, Ye W, Jiao C, Zhu L, Mao J, Jacob Tj, Wang L, Chen L. J Cell Physiol. 2011 Oct;226(10):2516-26. Doi: 10.1002/Jcp.22596. Clc-3 Is A Main Component Of Background Chloride Channels Activated Under Isotonic Conditions By Autocrine Atp In...
Xie S, Xie N, Li Y, Wang P, Zhang C, Li Q, Liu X, Deng J, Zhang C, Lv C. Mol Cell Biochem. 2012 Feb;361(1-2):305-14. Doi: 10.1007/S11010-011-1116-7. Epub 2011 Oct 20. Upregulation Of Trb2 Induced By Mir-98 In The Early Lesions Of Large Artery Of T...
Zhang Y, Yao W, Qiu J, Qian W, Zhu C, Zhang C. Neurosci Lett. 2011 Nov 14;505(2):71-5. Doi: 10.1016/J.Neulet.2011.09.055. Epub 2011 Oct 1. The Involvement Of Down-Regulation Of Cdh1-Apc In Hippocampal Neuronal Apoptosis After Global Cerebral Ische...
Zhu L, Yang H, Zuo W, Yang L, Zhang H, Ye W, Mao J, Chen L, Wang L. Biochem Pharmacol. 2012 Feb 1;83(3):324-34. Doi: 10.1016/J.Bcp.2011.11.007. Epub 2011 Nov 18. Differential Expression And Roles Of Volume-Activated Chloride Channels In Control Of...
Hu C, Zou Mj, Zhao L, Lu N, Sun Yj, Gou Sh, Xi T, Guo Ql. Toxicol Lett. 2012 Apr 5;210(1):78-86. Doi: 10.1016/J.Toxlet.2012.01.019. Epub 2012 Jan 31. E Platinum, A Newly Synthesized Platinum Compound, Induces Autophagy Via Inhibiting Phosphorylati...
Shi Jh, Hu Dh, Zhang Zf, Bai Xz, Wang Ht, Zhu Xx, Su Yj, Tang Cw. Arch Dermatol Res. 2012 Apr;304(3):209-15. Doi: 10.1007/S00403-012-1204-X. Epub 2012 Jan 12. Reduced Expression Of Microtubule-Associated Protein 1 Light Chain 3 In Hypertrophic Scars.
Zhang W, Li Xj, Zeng X, Shen Dy, Liu Cq, Zhang Hj, Xu Cb, Li Xy. Toxicol Lett. 2012 Mar 7;209(2):107-12. Doi: 10.1016/J.Toxlet.2011.12.005. Epub 2011 Dec 20. Activation Of Nuclear Factor-??b Pathway Is Responsible For Tumor Necrosis Factor-??-Induce...
Ji Yl, Wang Z, Wang H, Zhang C, Zhang Y, Zhao M, Chen Yh, Meng Xh, Xu Dx. Reprod Toxicol. 2012 Nov;34(3):357-63. Doi: 10.1016/J.Reprotox.2012.04.011. Epub 2012 May 5. Ascorbic Acid Protects Against Cadmium-Induced Endoplasmic Reticulum Stress And ...
Zhang C, Chen X, Zhu Rm, Zhang Y, Yu T, Wang H, Zhao H, Zhao M, Ji Yl, Chen Yh, Meng Xh, Wei W, Xu Dx. Toxicol Lett. 2012 Aug 3;212(3):229-40. Doi: 10.1016/J.Toxlet.2012.06.002. Epub 2012 Jun 12. Endoplasmic Reticulum Stress Is Involved In Hepatic...
Leng X, Wang X, Pang W, Zhan R, Zhang Z, Wang L, Gao X, Qian L. Cell Stress Chaperones. 2013 Jul;18(4):483-93. Doi: 10.1007/S12192-013-0404-4. Epub 2013 Jan 19. Evidence Of A Role For Both Anti-Hsp70 Antibody And Endothelial Surface Membrane Hsp70...
Deng X, Zhong Y, Gu L, Shen W, Guo J. Brain Res Bull. 2013 May;94:56-62. Doi: 10.1016/J.Brainresbull.2013.02.007. Epub 2013 Mar 7. Mir-21 Involve In Erk-Mediated Upregulation Of Mmp9 In The Rat Hippocampus Following Cerebral Ischemia.
Li Hj, Guo Lm, Yang Ll, Zhou Yc, Zhang Yj, Guo J, Xie Xj, Guo Gz. Toxicol Lett. 2013 Jun 20;220(1):35-43. Doi: 10.1016/J.Toxlet.2013.04.001. Epub 2013 Apr 6. Electromagnetic-Pulse-Induced Activation Of P38 Mapk Pathway And Disruption Of Blood-Reti...
Dai Q, Yin Y, Liu W, Wei L, Zhou Y, Li Z, You Q, Lu N, Guo Q. Int J Biochem Cell Biol. 2013 Jul;45(7):1468-78. Doi: 10.1016/J.Biocel.2013.04.015. Epub 2013 Apr 21. Two P53-Related Metabolic Regulators, Tigar And Sco2, Contribute To Oroxylin A-Medi...
Zhu X, Wu L, Qiao H, Han T, Chen S, Liu X, Jiang R, Wei Y, Feng D, Zhang Y, Ma Y, Zhang S, Zhang J. J Cell Biochem. 2013 Dec;114(12):2643-53. Doi: 10.1002/Jcb.24611. Autophagy Stimulates Apoptosis In Her2-Overexpressing Breast Cancers Treated By L...
Ma Yx, Guo Z, Sun T. Neurosci Lett. 2013 Aug 9;549:130-4. Doi: 10.1016/J.Neulet.2013.05.028. Epub 2013 May 25. Cgrp Inhibits Norepinephrine Induced Apoptosis With Restoration Of Bcl-2/Bax In Cultured Cardiomyocytes Of Rat.
Tao L, Fu R, Wang X, Yao J, Zhou Y, Dai Q, Li Z, Lu N, Wang W. Toxicol Lett. 2014 Jul 3;228(1):1-12. Doi: 10.1016/J.Toxlet.2014.04.002. Epub 2014 Apr 19. Ll-202, A Newly Synthesized Flavonoid, Inhibits Tumor Growth Via Inducing G(2)/M Phase Arrest...
Li Xf, Chen Dp, Ouyang Fz, Chen Mm, Wu Y, Kuang Dm, Zheng L. J Hepatol. 2015 Jan;62(1):131-9. Doi: 10.1016/J.Jhep.2014.08.023. Epub 2014 Aug 23. Increased Autophagy Sustains The Survival And Pro-Tumourigenic Effects Of Neutrophils In Human Hepatoc...
Zhou D, Wang Y, Tokunaga K, Huang F, Sun B, Yang R. Virus Res. 2015 Jan 2;195:25-34. Doi: 10.1016/J.Virusres.2014.08.021. Epub 2014 Sep 6. The Hiv-1 Accessory Protein Vpr Induces The Degradation Of The Anti-Hiv-1 Agent Apobec3G Through A Vprbp-Med...
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Tang H, Liu P, Yang L, Xie X, Ye F, Wu M, Liu X, Chen B, Zhang L, Xie X. Mol Cancer Ther. 2014 Dec;13(12):3185-97. Doi: 10.1158/1535-7163.Mct-14-0243. Epub 2014 Oct 15. Mir-185 Suppresses Tumor Proliferation By Directly Targeting E2F6 And Dnmt1 An...
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is gamma-actin antibody
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is gamma-actin antibody