Anti-Actinin Alpha 4 ACTN4 Antibody

Alpha Actinin 4 antibody

Boster Bio Anti-Actinin Alpha 4 ACTN4 Antibody catalog # A01975. Tested in WB,ICC,IF,IP,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Product Info Summary

SKU: A01975
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

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Product Name

Anti-Actinin Alpha 4 ACTN4 Antibody

View all Alpha Actinin 4 Antibodies

SKU/Catalog Number

A01975

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Actinin Alpha 4 ACTN4 Antibody catalog # A01975. Tested in WB,ICC,IF,IP,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Actinin Alpha 4 ACTN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01975)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MEK Kinase-1 around the phosphorylation site of T1402.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01975 is reactive to ACTN4 in Human, Mouse, Rat

Applications

A01975 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

104.854kDa

Background of Alpha Actinin 4

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
IP: 1:10-1:50

Validation Images & Assay Conditions

Gene/Protein Information For ACTN4 (Source: Uniprot.org, NCBI)

Gene Name

ACTN4

Full Name

Alpha-actinin-4

Weight

104.854kDa

Superfamily

alpha-actinin family

Alternative Names

actinin alpha4 isoform; actinin, alpha 4; ACTININ-4; alpha-actinin-4; DKFZp686K23158; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; FSGS; FSGS1; Non-muscle alpha-actinin 4 ACTN4 ACTININ-4, FSGS, FSGS1 actinin alpha 4 alpha-actinin-4|focal segmental glomerulosclerosis 1|non-muscle alpha-actinin 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTN4, check out the ACTN4 Infographic

ACTN4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A01975-100ul

100ul

$405

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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