Data & Images
|Product Name||Anti-ADAM10 Antibody|
|Description||Rabbit IgG polyclonal antibody for Disintegrin and metalloproteinase domain-containing protein 10(ADAM10) detection. Tested with WB in Human.|
|Cite This Product||Anti-ADAM10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1535)|
|Replacement Item||This antibody may replace the following items: sc-31853|sc-31855|sc-16523|sc-25578|sc-48400|sc-16524|sc-28358 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM10(612-627aa SVQWSRHFSGRTITLQ).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Disintegrin and metalloproteinase domain-containing protein 10(ADAM 10)|
|Molecular Weight||84142 MW|
|Protein Function|| Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling. .
|Tissue Specificity||Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. .|
|Sequence Similarities||Contains 1 disintegrin domain.|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.|
|Alternative Names||Disintegrin and metalloproteinase domain-containing protein 10;ADAM 10;18.104.22.168;CDw156;Kuzbanian protein homolog;Mammalian disintegrin-metalloprotease;CD156c;ADAM10;KUZ, MADM;|
|Research Areas|||cell biology|apoptosis|receptors|receptor processing| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|adam protein family| cancer|invasion/microenvironment| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|adams|cell death||
Background for Disintegrin and metalloproteinase domain-containing protein 10(ADAM 10)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ADAM10 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ADAM10 Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Human ADAM10 Protein 10ng
Lane 2: Recombinant Human ADAM10 Protein 5ng
Lane 3: Recombinant Human ADAM10 Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,