Data & Images
|Product Name||Anti-ADAMTS1 Antibody|
|Description||Rabbit IgG polyclonal antibody for A disintegrin and metalloproteinase with thrombospondin motifs 1(ADAMTS1) detection. Tested with WB in Mouse;Rat.|
|Cite This Product||Anti-ADAMTS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2071)|
|Replacement Item||This antibody may replace the following items: sc-25581|sc-31080|sc-47726|sc-47727|sc-52911|sc-5463|sc-5466|sc-5467 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse ADAMTS1(956-968aa KHYIDFCTLTQCS), different from the related rat sequence by one amino acid, and from the related human sequence by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||A disintegrin and metalloproteinase with thrombospondin motifs 1|
|Molecular Weight||105801 MW|
|Protein Function||Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. Has angiogenic inhibitor activity (By similarity). Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture (By similarity). .|
|Sequence Similarities||Contains 1 disintegrin domain.|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix.|
|Alternative Names||A disintegrin and metalloproteinase with thrombospondin motifs 1;ADAM-TS 1;ADAM-TS1;ADAMTS-1;3.4.24.-;Adamts1;|
|Research Areas|||cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|mmp| tags & cell markers|cell type markers|tumor associated| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes| cancer|invasion/microenvironment|adam protein family| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|adam ts||
Background for A disintegrin and metalloproteinase with thrombospondin motifs 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ADAMTS1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ADAMTS1 Antibody Images
Click the images to enlarge.
All lanes: Anti ADAMTS1 (PA2071) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 105KD
Observed bind size: 105KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,