Data & Images
|Product Name||Anti-ADAMTS5 Antibody|
|Description||Rabbit IgG polyclonal antibody for A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-ADAMTS5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2072)|
|Replacement Item||This antibody may replace the following items: sc-134952|sc-83186 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ADAMTS5(772-787aa TAYLALKKKNGEYLIN), different from the related mouse and rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||A disintegrin and metalloproteinase with thrombospondin motifs 5|
|Molecular Weight||101718 MW|
|Protein Function||Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.|
|Tissue Specificity||Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.|
|Sequence Similarities||Contains 1 disintegrin domain.|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix .|
|Alternative Names||A disintegrin and metalloproteinase with thrombospondin motifs 5;ADAM-TS 5;ADAM-TS5;ADAMTS-5;3.4.24.-;A disintegrin and metalloproteinase with thrombospondin motifs 11;ADAM-TS 11;ADAMTS-11;ADMP-2;Aggrecanase-2;ADAMTS5;ADAMTS11, ADMP2;|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|aggrecan| signal transduction|ecm enzymes|mmp|adam protein family| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|adam ts||
Background for A disintegrin and metalloproteinase with thrombospondin motifs 5
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ADAMTS5 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ADAMTS5 Antibody Images
Click the images to enlarge.
IHC(P): Rat Brain Tissue
All lanes: Anti ADAMTS5 (PA2072) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 102KD
Observed bind size: 102KD
IHC(P): Rat Brain Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,