Product Info Summary
SKU: | A05106-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Adducin Beta (E497) ADD2 Antibody
View all beta Adducin Antibodies
SKU/Catalog Number
A05106-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Adducin Beta (E497) ADD2 Antibody catalog # A05106-1. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Adducin Beta (E497) ADD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05106-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human IRPL1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05106-1 is reactive to ADD2 in Human, Mouse, Rat
Applications
A05106-1 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
80.854kDa
Background of beta Adducin
Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).
Miyata A., Biochem. Biophys. Res. Commun. 200:1728-1734(1994).
Chevalier D., Hum. Genet. 108:148-155(2001).
Deloukas P., Nature 414:865-871(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ADD2 using Anti-Adducin Beta (E497) ADD2 Antibody (A05106-1).
Immunohistochemistry (IHC) analyzes of Adducin β (E497) pAb in paraffin-embedded human breast carcinoma tissue at 1:50Click image to see more details
Figure 3. Immunohistochemistry validation of ADD2 using Anti-Adducin Beta (E497) ADD2 Antibody (A05106-1).
Immunohistochemistry (IHC) analyzes of Adducin β (E497) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50Click image to see more details
Figure 1. Western blotting validation for Anti-Adducin Beta (E497) ADD2 Antibody A05106-1
Western blot (WB) analysis of Adducin β (E497) pAb at 1:500 dilutionLane1:C6 whole cell lysate(40ug)
Lane2:The Brain tissue lysate of Mouse(40ug)
Lane3:U-87MG whole cell lysate(40ug)
Lane4:K562 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ADD2 (Source: Uniprot.org, NCBI)
Gene Name
ADD2
Full Name
Beta-adducin
Weight
80.854kDa
Superfamily
aldolase class II family
Alternative Names
ADDBErythrocyte adducin subunit beta; adducin 2 (beta); beta adducin; beta-adducin ADD2 ADDB adducin 2 beta-adducin|adducin 2 (beta)|erythrocyte adducin subunit beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADD2, check out the ADD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Adducin Beta (E497) ADD2 Antibody (A05106-1)
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No publications found for A05106-1
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