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SKU:PA2014-1
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:ELISA, IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Adiponectin Antibody
SKU/Catalog Number PA2014-1
Description Rabbit IgG polyclonal antibody for Adiponectin(ADIPOQ) detection. Tested with WB, IHC-P, IHC-F, ELISA in Human;Mouse;Rat.
Cite This Product Anti-Adiponectin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2014-1)
Replacement Item This antibody may replace the following items: sc-19822|sc-368665 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application ELISA, IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human ADIPOQ(224-244aa LYADNDNDSTFTGFLLYHDTN), different from the related mouse and rat sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ADIPOQ
Protein Name Adiponectin
Molecular Weight 26414 MW
Protein Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .
Tissue Specificity Synthesized exclusively by adipocytes and secreted into plasma.
Sequence Similarities Contains 1 C1q domain.
Subcellular Localization Secreted.
Uniprot ID Q15848
Alternative Names Adiponectin;30 kDa adipocyte complement-related protein;Adipocyte complement-related 30 kDa protein;ACRP30;Adipocyte, C1q and collagen domain-containing protein;Adipose most abundant gene transcript 1 protein;apM-1;Gelatin-binding protein;ADIPOQ;ACDC, ACRP30, APM1, GBP28;
Research Areas |cardiovascular|lipids / lipoproteins|adipose related|acrp| signal transduction|growth factors/hormones| neuroscience|neurology process|metabolism| stem cells|mesenchymal stem cells|adipogenesis| cardiovascular|atherosclerosis|diabetes associated| cancer|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease|diabetes|obesity|heart disease|metabolic disorders|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Adiponectin

ADIPOQ(Adipocyte-, C1q-, and Collagen Domain-Containing), also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al.(2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al.(1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue(and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

Anti-Adiponectin Antibody Images

Click the images to enlarge.

Anti-Adiponectin Antibody
Anti-Adiponectin antibody, PA2014-1, Western blotting
WB: Rat Heart Tissue Lysate
Anti-Adiponectin Antibody
Anti-Adiponectin antibody, PA2014-1, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human ADIPOQ, 44.4 KD (162aa tag+ M1-N244)
Lane 1: Recombinant Human ADIPOQ Protein 10ng
Lane 2: Recombinant Human ADIPOQ Protein 5ng
Lane 3: Recombinant Human ADIPOQ Protein 2.5ng
Anti-Adiponectin Antibody
Anti-Adiponectin antibody, PA2014-1, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-Adiponectin Antibody
Anti-Adiponectin antibody, PA2014-1, IHC(P)
IHC(P): Mouse Kidney Tissue
Anti-Adiponectin Antibody
Anti-Adiponectin antibody, PA2014-1, IHC(F)
IHC(F): Rat Liver Tissue
Anti-Adiponectin Antibody
Anti-Adiponectin antibody, PA2014-1, IHC(P)
IHC(P): Human Placenta Tissue
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Publications

Gao Y, Li F, Zhang Y, Dai L, Jiang H, Liu H, Zhang S, Chen C, Zhang J. Cell Physiol Biochem. 2013;31(2-3):452-61. Doi: 10.1159/000343381. Epub 2013 Mar 15. Silencing Of Adipoq Efficiently Suppresses Preadipocyte Differentiation In Porcine.
Liu F, Mu J, Yuan Z, Wu G, Liu E, Zheng S, Lian Q, Ren K, Xu H. Nutrition. 2012 Apr;28(4):422-5. Doi: 10.1016/J.Nut.2011.08.018. Epub 2011 Dec 20. High Salt Intake Fails To Enhance Plasma Adiponectin In Normotensive Salt-Sensitive Subjects.
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.