|Application:||ELISA, IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-Adiponectin Antibody|
|Description||Rabbit IgG polyclonal antibody for Adiponectin(ADIPOQ) detection. Tested with WB, IHC-P, IHC-F, ELISA in Human;Mouse;Rat.|
|Cite This Product||Anti-Adiponectin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2014-1)|
|Replacement Item||This antibody may replace the following items: sc-19822|sc-368665 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||ELISA, IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ADIPOQ(224-244aa LYADNDNDSTFTGFLLYHDTN), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||26414 MW|
|Protein Function||Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .|
|Tissue Specificity||Synthesized exclusively by adipocytes and secreted into plasma.|
|Sequence Similarities||Contains 1 C1q domain.|
|Alternative Names||Adiponectin;30 kDa adipocyte complement-related protein;Adipocyte complement-related 30 kDa protein;ACRP30;Adipocyte, C1q and collagen domain-containing protein;Adipose most abundant gene transcript 1 protein;apM-1;Gelatin-binding protein;ADIPOQ;ACDC, ACRP30, APM1, GBP28;|
|Research Areas|||cardiovascular|lipids / lipoproteins|adipose related|acrp| signal transduction|growth factors/hormones| neuroscience|neurology process|metabolism| stem cells|mesenchymal stem cells|adipogenesis| cardiovascular|atherosclerosis|diabetes associated| cancer|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease|diabetes|obesity|heart disease|metabolic disorders||
Background for Adiponectin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Adiponectin Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
ELISA , 0.1-0.5μg/ml, Human, -|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Adiponectin Antibody Images
Click the images to enlarge.
WB: Rat Heart Tissue Lysate
Recombinant Protein Detection Source: E.coli derived -recombinant Human ADIPOQ, 44.4 KD (162aa tag+ M1-N244)
Lane 1: Recombinant Human ADIPOQ Protein 10ng
Lane 2: Recombinant Human ADIPOQ Protein 5ng
Lane 3: Recombinant Human ADIPOQ Protein 2.5ng
IHC(P): Rat Kidney Tissue
IHC(P): Mouse Kidney Tissue
IHC(F): Rat Liver Tissue
IHC(P): Human Placenta Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,