Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody

Mouse IgG monoclonal antibody for AFP, alpha-fetoprotein (AFP) detection. Tested with IHC-P in Human. No cross reactivity with other proteins.

SKU MA1001
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number C3
Host Mouse
Ig Isotype Mouse IgG2a
Applications IHC

Overview

Product Name Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody
See all Afp primary antibodies, ELISA kits and proteins
SKU/Catalog Number MA1001
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Monoclonal clone# G2 antibody for ALPHA 1 FETOPROTEIN/AFP detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. ALPHA 1 FETOPROTEIN/AFP information: Molecular Weight: 68386 MW; Subcellular Localization: Isoform 1: Secreted; Tissue Specificity: Plasma.
Cite This Product Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1001)
Specificity Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody (MA1001) reacts with Human Afp, in native form and recombinant. Superfamily members of Afp are not reactive to MA1001.
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunogen Human alpha-fetoprotein.
Reactivity Human

Assay Details

Our Boster Quality Guarantee for Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, By Heat

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody (MA1001).

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1001-1-IHC-anti-afp-alpha-1-fetoprotein-antibody.jpg

Anti-AFP antibody (monoclonal), MA1001, IHC(P)
IHC(P): Human Liver Cancer Tissue

/antibody/ma1001-2-IHC-anti-afp-alpha-1-fetoprotein-antibody.jpg

Anti-AFP antibody (monoclonal), MA1001, IHC(P)
IHC(P): Human Hepatitis Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P02773
Gene Name Afp
Protein Name Alpha-fetoprotein
Tissue Specificity Plasma. .
Alternative Names Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;Afp;
Subcellular Localization Isoform 1: Secreted.
Molecular Weight 68386 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Binds estrogens, fatty acids and metals.
Research Areas Human

*You can search these to find other products in these research areas.
Background Alpha-fetoprotein(AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. Direct confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk(1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.

Other Recommended Resources

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Publishing with Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody (MA1001)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]

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In stock
Order Product
MA1001
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.
Adipose tissue-derived mesenchymal stem cells differentiated into hepatocyte-like cellsin vivoandin vitro
Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells
Shan Zy, Wu Ys, Li X, Shen Xh, Wang Zd, Liu Zh, Shen Jl, Lei L. Cell Reprogram. 2014 Feb;16(1):77-83. Doi: 10.1089/Cell.2013.0067. Epub 2014 Jan 4. Continuous Passages Accelerate The Reprogramming Of Mouse Induced Pluripotent Stem Cells.
Su Z, Wu R, Tan Z, Li Y, Chen L, Luo J, Zhang M. Biochem Biophys Res Commun. 2010 Apr 9;394(3):616-22. Doi: 10.1016/J.Bbrc.2010.03.033. Epub 2010 Mar 7. Early Homing Behavior Of Stro-1- Mesenchyme-Like Cells Derived From Human Embryonic Stem Cells...
Yin L, Zhu Y, Yang J, Ni Y, Zhou Z, Chen Y, Wen L. Mol Med Rep. 2015 Mar;11(3):1722-32. Doi: 10.3892/Mmr.2014.2935. Epub 2014 Nov 13. Adipose Tissue-Derived Mesenchymal Stem Cells Differentiated Into Hepatocyte-Like Cells In Vivo And In Vitro.
Li Y, Wu Z, Liu Z. Analyst. 2015 Jun 21;140(12):4083-8. Doi: 10.1039/C5An00357A. Epub 2015 Apr 17. An Immune Sandwich Assay Of Carcinoembryonic Antigen Based On The Joint Use Of Upconversion Phosphors And Magnetic Beads.
Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to afp antibody, alpha fetoprotein antibody, alpha-1-fetoprotein antibody, alpha-fetoprotein antibody
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