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SKU:MA1001
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:C3
Application:IHC-P
Price: $200.00
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Overview

Product Name Anti-AFP/Alpha 1 Fetoprotein Antibody
SKU/Catalog Number MA1001
Description Mouse IgG monoclonal antibody for AFP, alpha-fetoprotein (AFP) detection. Tested with IHC-P in Human. No cross reactivity with other proteins.
Cite This Product Anti-AFP/Alpha 1 Fetoprotein Antibody C3 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1001)
Replacement Item This antibody may replace the following items: sc-166335|sc-15375|sc-166325|sc-166450|sc-8399|sc-8108|sc-69857|sc-51506|sc-130302|sc-80464 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2a
Validated Species Human
Application IHC-P

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human alpha-fetoprotein.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number C3
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Afp
Protein Name Alpha-fetoprotein
Molecular Weight 68386 MW
Protein Function Binds estrogens, fatty acids and metals.
Tissue Specificity Plasma. .
Sequence Similarities Belongs to the ALB/AFP/VDB family.
Subcellular Localization Isoform 1: Secreted.
Uniprot ID P02773
Alternative Names Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;Afp;
Research Areas |cardiovascular|blood|serum proteins| cancer|tumor biomarkers|tumor antigens| developmental biology|organogenesis|gut development|liver development| kits/ lysates/ other|elisa kits|cardiovascular elisa kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Alpha-fetoprotein

Alpha-fetoprotein(AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. Direct confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk(1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.

Anti-AFP/Alpha 1 Fetoprotein Antibody Images

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Anti-AFP/Alpha 1 Fetoprotein Antibody
Anti-AFP antibody (monoclonal), MA1001, IHC(P)
IHC(P): Human Liver Cancer Tissue
Anti-AFP/Alpha 1 Fetoprotein Antibody
Anti-AFP antibody (monoclonal), MA1001, IHC(P)
IHC(P): Human Hepatitis Tissue
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Publications

Mikulits, W., & Reichl, P. (2017). US Patent Application Publication No. US 2017/0146540 A1. Washington, DC: US Patent and Trademark Office.
Shan Zy, Wu Ys, Li X, Shen Xh, Wang Zd, Liu Zh, Shen Jl, Lei L. Cell Reprogram. 2014 Feb;16(1):77-83. Doi: 10.1089/Cell.2013.0067. Epub 2014 Jan 4. Continuous Passages Accelerate The Reprogramming Of Mouse Induced Pluripotent Stem Cells.
Su Z, Wu R, Tan Z, Li Y, Chen L, Luo J, Zhang M. Biochem Biophys Res Commun. 2010 Apr 9;394(3):616-22. Doi: 10.1016/J.Bbrc.2010.03.033. Epub 2010 Mar 7. Early Homing Behavior Of Stro-1- Mesenchyme-Like Cells Derived From Human Embryonic Stem Cells...
Yin L, Zhu Y, Yang J, Ni Y, Zhou Z, Chen Y, Wen L. Mol Med Rep. 2015 Mar;11(3):1722-32. Doi: 10.3892/Mmr.2014.2935. Epub 2014 Nov 13. Adipose Tissue-Derived Mesenchymal Stem Cells Differentiated Into Hepatocyte-Like Cells In Vivo And In Vitro.
Li Y, Wu Z, Liu Z. Analyst. 2015 Jun 21;140(12):4083-8. Doi: 10.1039/C5An00357A. Epub 2015 Apr 17. An Immune Sandwich Assay Of Carcinoembryonic Antigen Based On The Joint Use Of Upconversion Phosphors And Magnetic Beads.
Wu R, Gu B, Zhao X, Tan Z, Chen L, Zhu J, Zhang M. Hum Cell. 2013 Mar;26(1):19-27. Doi: 10.1007/S13577-011-0022-3. Epub 2011 Jun 15. Derivation Of Multipotent Nestin(+)/Cd271 (-)/Stro-1 (-) Mesenchymal-Like Precursors From Human Embryonic Stem Cel...
Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...
Qin Xg, Hua Z, Shuang W, Wang Yh, Cui Yd. Pharm Biol. 2010 Mar;48(3):275-81. Doi: 10.3109/13880200903104101. Effects Of Matrine On Hepg2 Cell Proliferation And Expression Of Tumor Relevant Proteins In Vitro.
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to afp antibody, alpha fetoprotein antibody, alpha-1-fetoprotein antibody, alpha-fetoprotein antibody