Anti-AGPAT5/Lpaat Epsilon Antibody

Agpat5 antibody

Boster Bio Anti-AGPAT5/Lpaat Epsilon Antibody (Catalog # A10870). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A10870
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-AGPAT5/Lpaat Epsilon Antibody

View all Agpat5 Antibodies

SKU/Catalog Number

A10870

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-AGPAT5/Lpaat Epsilon Antibody (Catalog # A10870). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AGPAT5/Lpaat Epsilon Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10870)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human AGPAT5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10870 is reactive to Agpat5 in Human

Applications

A10870 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42.202kDa

Background of Agpat5

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor. Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA. Activity toward lysophosphatidylglycerol not detectable.

Wiemann S., Genome Res. 11:422-435(2001).
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Agpat5 (Source: Uniprot.org, NCBI)

Gene Name

Agpat5

Full Name

1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon

Weight

42.202kDa

Superfamily

1-acyl-sn-glycerol-3-phosphate acyltransferase family

Alternative Names

1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acidacyltransferase, epsilon); 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon; 1-AGPAT 5,1-acylglycerol-3-phosphate O-acyltransferase 5; 1AGPAT5,1-AGP acyltransferase 5; EC 2.3.1.51; FLJ11210; LPAATE; LPAAT-e; LPAAT-epsilon; Lysophosphatidic acid acyltransferase epsilon Agpat5|1110013A05Rik, D8Ertd319, D8Ertd319e|1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon)|1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon|1-AGP acyltransferase 5|1-AGPAT 5|LPAAT-epsilon|lysophosphatidic acid acyltransferase epsilon

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Agpat5, check out the Agpat5 Infographic

Agpat5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Agpat5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A10870

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-AGPAT5/Lpaat Epsilon Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-AGPAT5/Lpaat Epsilon Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Anti-AGPAT5/Lpaat Epsilon Antibody

Question

Here is the WB image, lot number and protocol we used for brain lung using anti-AGPAT5/Lpaat Epsilon antibody A10870. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-31

Question

We are currently using anti-AGPAT5/Lpaat Epsilon antibody A10870 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

The anti-AGPAT5/Lpaat Epsilon antibody (A10870) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-26

Question

My question regarding product A10870, anti-AGPAT5/Lpaat Epsilon antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

N. Gonzalez

Verified customer

Asked: 2014-09-08

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10870 anti-AGPAT5/Lpaat Epsilon antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-09-08

Order DetailsPrice
A10870-100uL

100uL

$360

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A10870
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.