Anti-AKAP2 Antibody Picoband™

Akap2 antibody

Boster Bio Anti-AKAP2 Antibody Picoband™ catalog # A09279. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A09279
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-AKAP2 Antibody Picoband™

View all Akap2 Antibodies

SKU/Catalog Number

A09279

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-AKAP2 Antibody Picoband™ catalog # A09279. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AKAP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09279)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human AKAP2, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09279 is reactive to Akap2 in Human, Mouse

Applications

A09279 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

150-170 kDa

Calculated molecular weight

95.941kDa

Background of Akap2

A-kinase anchor protein 2 is an enzyme that in humans is encoded by the AKAP2 gene. It is mapped to 9q31.3. The protein encoded by this gene binds to the regulatory subunit of protein kinase A and is found associated with the actin cytoskeleton. The encoded protein mediates signals carried by cAMP and may be involved in creating polarity in certain signaling processes. Three transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Akap2 (Source: Uniprot.org, NCBI)

Gene Name

Akap2

Full Name

A-kinase anchor protein 2

Weight

95.941kDa

Alternative Names

A kinase (PRKA) anchor protein 2; AKAP-2; FLJ53197; protein kinase A2 Akap2|AKAP-2|A-kinase anchoring protein 2|A-kinase anchor protein 2|A kinase (PRKA) anchor protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Akap2, check out the Akap2 Infographic

Akap2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Akap2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-AKAP2 Antibody Picoband™

Question

We are currently using anti-AKAP2 antibody A09279 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

The anti-AKAP2 antibody (A09279) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-14

Question

Is a blocking peptide available for product anti-AKAP2 antibody (A09279)?

M. Evans

Verified customer

Asked: 2019-12-23

Answer

We do provide the blocking peptide for product anti-AKAP2 antibody (A09279). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-23

Question

Will A09279 anti-AKAP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

As indicated on the product datasheet, A09279 anti-AKAP2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-10

Order DetailsPrice
A09279

100μg

$370
A09279-10ug

10μg sample (liquid)

$99
A09279-Biotin

100 μg Biotin conjugated

$570
A09279-Cy3

100 μg Cy3 conjugated

$570
A09279-Dylight488

100 μg Dylight488 conjugated

$570
A09279-Dylight550

100 μg Dylight550 conjugated

$570
A09279-Dylight594

100 μg Dylight594 conjugated

$570
A09279-FITC

100 μg FITC conjugated

$570
A09279-HRP

100 μg HRP conjugated

$570
A09279-APC

100 μg APC conjugated

$670
A09279-PE

100 μg PE conjugated

$670
A09279-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A09279
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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