Product Info Summary
SKU: | A04234-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AKAP5 (E7) Antibody
SKU/Catalog Number
A04234-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AKAP5 (E7) Antibody catalog # A04234-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AKAP5 (E7) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04234-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human C1q-B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04234-1 is reactive to AKAP5 in Human, Mouse, Rat
Applications
A04234-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
47.088kDa
Background of AKAP5
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Reid K.B.M.; Biochem. J. 231:729-735(1985).
Gregory S.G., Nature 441:315-321(2006).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of AKAP5 using Anti-AKAP5 (E7) Antibody (A04234-1).
Immunohistochemistry (IHC) analyzes of AKAP5 (E7) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-AKAP5 (E7) Antibody A04234-1
Western blot (WB) analysis of AKAP5 (E7) pAb at 1:500 dilutionLane1:MEF whole cell lysate(40ug)
Lane2:C6 whole cell lysate(40ug)
Lane3:Hela whole cell lysate(40ug)
Lane4:A549 whole cell lysate(40ug)
Lane5:HEK293T whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For AKAP5 (Source: Uniprot.org, NCBI)
Gene Name
AKAP5
Full Name
A-kinase anchor protein 5
Weight
47.088kDa
Alternative Names
A kinase (PRKA) anchor protein 5; AKAP 79; AKAP7979kDa; A-kinase anchor protein 79 kDa; A-kinase anchoring protein 75/79; cAMP-dependent protein kinase regulatory subunit II high affinity bindingprotein; cAMP-dependent protein kinase regulatory subunit II high affinity-bindingprotein; H21 AKAP5 AKAP75, AKAP79, H21 A-kinase anchoring protein 5 A-kinase anchor protein 5|A kinase (PRKA) anchor protein 5|A-kinase anchor protein 79 kDa|A-kinase anchoring protein 75/79|AKAP 79|cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKAP5, check out the AKAP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKAP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKAP5 (E7) Antibody (A04234-1)
Hello CJ!
No publications found for A04234-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AKAP5 (E7) Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-AKAP5 (E7) Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question