Data & Images
|Product Name||Anti-ALDH3A1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Aldehyde dehydrogenase, dimeric NADP-preferring(ALDH3A1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-ALDH3A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2074)|
|Replacement Item||This antibody may replace the following items: sc-54057|sc-67309|sc-54055|sc-54054|sc-67310|sc-376089|sc-137168|sc-54058|sc-54053|sc-373688|sc-54059|sc-514043 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ALDH3A1(440-453aa KVRYPPSPAKMTQH).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Aldehyde dehydrogenase, dimeric NADP-preferring|
|Molecular Weight||50395 MW|
|Protein Function||ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.|
|Tissue Specificity||High levels in stomach, esophagus and lung; low level in the liver and kidney.|
|Alternative Names||Aldehyde dehydrogenase, dimeric NADP-preferring;18.104.22.168;ALDHIII;Aldehyde dehydrogenase 3;Aldehyde dehydrogenase family 3 member A1;ALDH3A1;ALDH3;|
|Research Areas|||signal transduction|metabolism|energy metabolism| neuroscience|sensory system|visual system| signal transduction|alcohol metabolism| cell biology|other antibodies|oxidative stress| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|redox metabolism|types of disease|cancer||
Background for Aldehyde dehydrogenase, dimeric NADP-preferring
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ALDH3A1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ALDH3A1 Antibody Images
Click the images to enlarge.
All lanes: Anti ALDH3A1 (PA2074) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
IHC(P): Human Gastric Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,