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SKU:PA1004-2
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-Alkaline Phosphatase Antibody
SKU/Catalog Number PA1004-2
Description Rabbit IgG polyclonal antibody for Alkaline phosphatase, tissue-nonspecific isozyme(ALPL) detection. Tested with WB in Human.
Cite This Product Anti-Alkaline Phosphatase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1004-2)
Replacement Item This antibody may replace the following items: sc-137213|sc-166261|sc-21707|sc-21708|sc-23430|sc-30203|sc-73524|sc-73525|sc-81754 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Alkaline Phosphatase(31-45aa DQAQETLKYALELQK), different from the related mouse and rat sequences by three amino acids.
Cross Reactivity No cross reactivity with other proteins.
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ALPL
Protein Name Alkaline phosphatase, tissue-nonspecific isozyme
Molecular Weight 57305 MW
Protein Function This isozyme may play a role in skeletal mineralization.
Subcellular Localization Cell membrane ; Lipid-anchor, GPI-anchor .
Uniprot ID P05186
Alternative Names Alkaline phosphatase, tissue-nonspecific isozyme;AP-TNAP;TNSALP;3.1.3.1 ;Alkaline phosphatase liver/bone/kidney isozyme;ALPL;
Research Areas |cell biology|cell cycle|kinases/phosphatases| tags & cell markers|fusion / marker proteins|alkaline phosphatase| cancer|tumor biomarkers|enzymes|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Alkaline phosphatase, tissue-nonspecific isozyme

Alkaline phosphatase(ALPL) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific(found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts,the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb.Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Anti-Alkaline Phosphatase Antibody Images

Click the images to enlarge.

Anti-Alkaline Phosphatase Antibody
All lanes: Anti ALPL (PA1004-2) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
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Publications

Liu B, Deng C, Zhang Y, Zhang J. Neural Regen Res. 2012 Jul 5;7(19):1463-8. Doi: 10.3969/J.Issn.1673-5374.2012.19.003. Wnt3A Expression During The Differentiation Of Adipose-Derived Stem Cells Into Cholinergic Neurons.
Su Y, Wang F, Qi H, Zhao Sg, Li X, Cui H. Mol Vis. 2011 Jan 22;17:247-56. Small Interfering Rna Targeting Of S Phase Kinase-Interacting Protein 2 Inhibits Cell Proliferation Of Pterygium Fibroblasts.
Li Q, Xia Yy, Tang Jc, Wang Ry, Bei Cy, Zeng Y. Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42. Doi: 10.3109/10731199.2010.502880. Epub 2010 Jul 23. In Vitro And In Vivo Biocompatibility Investigation Of Diamond-Like Carbon Co...
Zhao Jw, Gao Zl, Mei H, Li Yl, Wang Y. Am J Med Sci. 2011 Jun;341(6):460-8. Doi: 10.1097/Maj.0B013E31820865D5. Differentiation Of Human Mesenchymal Stem Cells: The Potential Mechanism For Estrogen-Induced Preferential Osteoblast Versus Adipocyte D...
Zhang P, Wu X, Hu C, Wang P, Li X. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):30-6. Doi: 10.1007/S11626-011-9471-Y. Epub 2011 Dec 17. Rho Kinase Inhibitor Y-27632 And Accutase Dramatically Increase Mouse Embryonic Stem Cell Derivation.
Shen Z, Ren Y, Ye D, Guo J, Kang C, Ding H. Eur J Histochem. 2011 Mar 21;55(1):E9. Doi: 10.4081/Ejh.2011.E9. Significance And Relationship Between Dj-1 Gene And Surviving Gene Expression In Laryngeal Carcinoma.
Lu Gh, Song Wt, Wang C, Yan Zh. Chemosphere. 2010 Aug;80(9):982-90. Doi: 10.1016/J.Chemosphere.2010.05.038. Epub 2010 Jun 19. Assessment Of In Vivo Estrogenic Response And The Identification Of Environmental Estrogens In The Yangtze River (Nanjing...
Shen Z, Zhan G, Deng H, Ren Y, Ye D, Xiao B, Guo J. J Laryngol Otol. 2013 Dec;127(12):1194-202. Doi: 10.1017/S0022215113003174. Epub 2013 Dec 3. Microrna-519A Demonstrates Significant Tumour Suppressive Activity In Laryngeal Squamous Cells By Targ...
Liu J, Lu G, Wu D, Yan Z. Ecotoxicol Environ Saf. 2014 Apr;102:12-7. Doi: 10.1016/J.Ecoenv.2014.01.014. Epub 2014 Feb 1. A Multi-Biomarker Assessment Of Single And Combined Effects Of Norfloxacin And Sulfamethoxazole On Male Goldfish (Carassius Au...
Li M, Yan Z, Han W, Zhang Y. Cell Immunol. 2006 Feb;239(2):136-43. Epub 2006 Jun 30. Mimotope Vaccination For Epitope-Specific Induction Of Anti-Cd20 Antibodies.
Guan W, Wang Y, Hou L, Chen L, Li X, Yue W, Ma Y. Poult Sci. 2010 Feb;89(2):312-7. Doi: 10.3382/Ps.2009-00413. Derivation And Characteristics Of Pluripotent Embryonic Germ Cells In Duck.
Lai G, Yan F, Wu J, Leng C, Ju H. Anal Chem. 2011 Apr 1;83(7):2726-32. Doi: 10.1021/Ac103283P. Epub 2011 Mar 3. Ultrasensitive Multiplexed Immunoassay With Electrochemical Stripping Analysis Of Silver Nanoparticles Catalytically Deposited By Gold ...
Wang W, Huang B, Feng Zg, Chen Xp, Tang Wx, Chen Zw. Z Naturforsch C. 2010 Nov-Dec;65(11-12):726-32. Expression, Purification, And Characterization Of Functional Recombinant Human Daintain/Aif-1 In Escherichia Coli.
Zhang Y, Yang J, Jiang S, Fang C, Xiong L, Cheng H, Xia Y. J Clin Immunol. 2012 Dec;32(6):1270-8. Doi: 10.1007/S10875-012-9724-X. Epub 2012 Jun 24. The Lupus-Derived Anti-Double-Stranded Dna Igg Contributes To Myofibroblast-Like Phenotype In Mesan...
Guo P, Shi Zl, Liu A, Lin T, Bi Fg, Shi Mm, Yan Sg. Int J Mol Sci. 2014 Nov 5;15(11):20117-33. Doi: 10.3390/Ijms151120117. Cartilage Oligomeric Matrix Protein Gene Multilayers Inhibit Osteogenic Differentiation And Promote Chondrogenic Differentia...
Song Wt, Lu Gh, Wang C, Zhang Hz, Xu S, Qin J. Bull Environ Contam Toxicol. 2010 Apr;84(4):406-12. Doi: 10.1007/S00128-010-9944-9. Epub 2010 Mar 6. Study On Environmental Estrogen Pollution In Yangtze River (Nanjing Section) By An In Vivo Bioassay.
Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A, Huang S. Mol Med Rep. 2014 Oct;10(4):1935-41. Doi: 10.3892/Mmr.2014.2430. Epub 2014 Jul 30. Decreased Osteoclastogenesis, Osteoblastogenesis And Low Bone Mass In A Mouse Model Of Type 2 Dia...
Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to alp antibody, alppl2 antibody, placental alkaline phosphatase antibody, plap antibody