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SKU:PB9073
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-ALOX15 Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Arachidonate 15-lipoxygenase(ALOX15) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-ALOX15 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9073)
Replacement Item This antibody may replace the following items: sc-133085|sc-27354|sc-32940 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ALOX15
Protein Name Arachidonate 15-lipoxygenase
Molecular Weight 74804 MW
Protein Function Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass. .
Tissue Specificity Detected in monocytes and eosinophils (at protein level). Expressed in airway epithelial cells. .
Sequence Similarities Belongs to the lipoxygenase family.
Subcellular Localization Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Lipid droplet. Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells.
Uniprot ID P16050
Alternative Names Arachidonate 15-lipoxygenase;15-LOX;15-LOX-1;1.13.11.33;12/15-lipoxygenase;Arachidonate 12-lipoxygenase, leukocyte-type;12-LOX;1.13.11.31;Arachidonate omega-6 lipoxygenase;ALOX15;LOG15;
Research Areas |signal transduction|growth factors/hormones| cancer|tumor biomarkers| cardiovascular|atherosclerosis|lipoprotein metabolism|lipids / lipoproteins|fatty acids| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|heart disease|redox metabolism|fatty acid oxidation|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Arachidonate 15-lipoxygenase

Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.

Anti-ALOX15 Picoband™ Antibody Images

Click the images to enlarge.

Anti-ALOX15 Picoband™ Antibody
Anti-ALOX15 Picoband antibody, PB9073-1.JPG
IHC(P): Mouse Lung Tissue
Anti-ALOX15 Picoband™ Antibody
Anti-ALOX15 Picoband antibody, PB9073-2.JPG
IHC(P): Rat Lung Tissue
Anti-ALOX15 Picoband™ Antibody
Anti-ALOX15 Picoband antibody, PB9073-3.JPG
IHC(P): Human Intestine Cancer Tissue
Anti-ALOX15 Picoband™ Antibody
Anti-ALOX15 Picoband antibody, PB9073-4.JPG
IHC(P): Human Mammary Cancer Tissue
Anti-ALOX15 Picoband™ Antibody
Anti-ALOX15 Picoband antibody, PB9073-5.jpg
All lanes: Anti-ALOX15(PB9073) at 0.5ug/m
WB: Recombinant Human ALOX15 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to 15 lipoxygenase 1 antibody, alox15 antibody