Data & Images
|Product Name||Anti-ALOX15 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Arachidonate 15-lipoxygenase(ALOX15) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-ALOX15 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9073)|
|Replacement Item||This antibody may replace the following items: sc-133085|sc-27354|sc-32940 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Arachidonate 15-lipoxygenase|
|Molecular Weight||74804 MW|
|Protein Function||Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass. .|
|Tissue Specificity||Detected in monocytes and eosinophils (at protein level). Expressed in airway epithelial cells. .|
|Sequence Similarities||Belongs to the lipoxygenase family.|
|Subcellular Localization||Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Lipid droplet. Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells.|
|Alternative Names||Arachidonate 15-lipoxygenase;15-LOX;15-LOX-1;220.127.116.11;12/15-lipoxygenase;Arachidonate 12-lipoxygenase, leukocyte-type;12-LOX;18.104.22.168;Arachidonate omega-6 lipoxygenase;ALOX15;LOG15;|
|Research Areas|||signal transduction|growth factors/hormones| cancer|tumor biomarkers| cardiovascular|atherosclerosis|lipoprotein metabolism|lipids / lipoproteins|fatty acids| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|heart disease|redox metabolism|fatty acid oxidation||
Background for Arachidonate 15-lipoxygenase
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ALOX15 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ALOX15 Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Mouse Lung Tissue
IHC(P): Rat Lung Tissue
IHC(P): Human Intestine Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
All lanes: Anti-ALOX15(PB9073) at 0.5ug/m
WB: Recombinant Human ALOX15 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,