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SKU:RP1031
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-ALOX5/5 Lo Antibody
SKU/Catalog Number RP1031
Description Rabbit IgG polyclonal antibody for Arachidonate 5-lipoxygenase(ALOX5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-ALOX5/5 Lo Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1031)
Replacement Item This antibody may replace the following items: sc-160317|sc-160316|sc-160318|sc-133547|sc-514522 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human ALOX5 recombinant protein (Position: A120-R483). Human ALOX5 shares 94% amino acid (aa) sequence identity with both mouse and rat ALOX5.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ALOX5
Protein Name Arachidonate 5-lipoxygenase
Molecular Weight 77983 MW
Protein Function Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. .
Sequence Similarities Belongs to the lipoxygenase family.
Subcellular Localization Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.
Uniprot ID P09917
Alternative Names Arachidonate 5-lipoxygenase;5-LO;5-lipoxygenase;1.13.11.34;ALOX5;LOG5;
Research Areas |signal transduction|metabolism|energy metabolism| cardiovascular|atherosclerosis|lipoprotein metabolism| cancer|cancer metabolism|metabolic signaling pathway|hormone biosynthesis| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|energy transfer pathways|endocrine metabolism|types of disease|heart disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Arachidonate 5-lipoxygenase

Arachidonate 5-lipoxygenase, also known as 5-LOX or 5-LO, is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes. It is mapped to 10q11.21. ALOX5 plays a dual role in the synthesis of leukotrienes from arachidonic acid. The position of ALOX5 within the nucleus of resting cells determines the capacity to generate LTB4 upon subsequent activation. It is involved in lung vascular tone regulation and in the development of chronic pulmonary hypertension in hypoxic rodent models. ALOX5 also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases.

Anti-ALOX5/5 Lo Antibody Images

Click the images to enlarge.

Anti-ALOX5/5 Lo Antibody
Anti-ALOX5 Picoband antibody, RP1031-1.JPG
IHC(P): Mouse Brain Tissue
Anti-ALOX5/5 Lo Antibody
Anti-ALOX5 Picoband antibody, RP1031-2.JPG
IHC(P): Rat Brain Tissue
Anti-ALOX5/5 Lo Antibody
Anti-ALOX5 Picoband antibody, RP1031-3.JPG
IHC(P): Human Intestinal Cancer Tissue
Anti-ALOX5/5 Lo Antibody
Anti-ALOX5 Picoband antibody, RP1031-4.JPG
IHC(P): Human Mammary Cancer Tissue
Anti-ALOX5/5 Lo Antibody
Anti-ALOX5 Picoband antibody, RP1031-5.jpg
All lanes: Anti-ALOX5 (RP1031) at 0.5ug/ml
WB: SGC Whole Cell Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is 5-lipoxygenase antibody