Anti-Alpha A Crystallin Monoclonal Antibody

AlphaA Crystallin/CRYAA antibody

Boster Bio Anti-Alpha A Crystallin Monoclonal Antibody catalog # M01900. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01900
Size: 200ug
Reactive Species: Bovine, Human, Mouse, Rat
Host: Mouse
Application: ELISA, IF, ICC, WB

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Product Name

Anti-Alpha A Crystallin Monoclonal Antibody

View all AlphaA Crystallin/CRYAA Antibodies

SKU/Catalog Number

M01900

Size

200ug

Form

liquid

Description

Boster Bio Anti-Alpha A Crystallin Monoclonal Antibody catalog # M01900. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha A Crystallin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01900)

Host

Mouse

Contents

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

1H3.B8

Isotype

IgG1

Immunogen

Native Human Alpha Crystallin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01900 is reactive to CRYAA in Bovine, Human, Mouse, Rat

Applications

M01900 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

19.909kDa

Background of AlphaA Crystallin/CRYAA

The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For CRYAA (Source: Uniprot.org, NCBI)

Gene Name

CRYAA

Full Name

Alpha-crystallin A chain

Weight

19.909kDa

Superfamily

small heat shock protein (HSP20) family

Alternative Names

AlphaA Crystallin; CRYA1; CRYA1crystallin, alpha-1; CRYAA; crystallin, alpha A; Heat shock protein beta-4; HSPB4; HSPB4alpha-crystallin A chain; human alphaA-crystallin (CRYA1), 10HspB4 CRYAA CRYA1, CTRCT9, HSPB4 crystallin alpha A alpha-crystallin A chain|crystallin, alpha-1|heat shock protein beta-4|human alphaA-crystallin (CRYA1)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CRYAA, check out the CRYAA Infographic

CRYAA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CRYAA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Alpha A Crystallin Monoclonal Antibody

Question

Would anti-Alpha A Crystallin Monoclonal antibody M01900 work on primate IF with spleen?

Verified Customer

Verified customer

Asked: 2019-03-18

Answer

Our lab technicians have not tested anti-Alpha A Crystallin Monoclonal antibody M01900 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Alpha A Crystallin Monoclonal antibody M01900 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate spleen in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-03-18

Question

Is a blocking peptide available for product anti-Alpha A Crystallin Monoclonal antibody (M01900)?

L. Walker

Verified customer

Asked: 2016-03-30

Answer

We do provide the blocking peptide for product anti-Alpha A Crystallin Monoclonal antibody (M01900). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-03-30

Question

I see that the anti-Alpha A Crystallin Monoclonal antibody M01900 works with WB, what is the protocol used to produce the result images on the product page?

R. Miller

Verified customer

Asked: 2015-08-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-08-20

Question

I have a question about product M01900, anti-Alpha A Crystallin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Wu

Verified customer

Asked: 2013-03-08

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01900 anti-Alpha A Crystallin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-03-08

Order DetailsPrice
M01900

200ug

$372

Free Secondary Antibody

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M01900
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$372.00

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