Product Info Summary
SKU: | M01900 |
---|---|
Size: | 200ug |
Reactive Species: | Bovine, Human, Mouse, Rat |
Host: | Mouse |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Alpha A Crystallin Monoclonal Antibody
View all AlphaA Crystallin/CRYAA Antibodies
SKU/Catalog Number
M01900
Size
200ug
Form
liquid
Description
Boster Bio Anti-Alpha A Crystallin Monoclonal Antibody catalog # M01900. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha A Crystallin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01900)
Host
Mouse
Contents
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
1H3.B8
Isotype
IgG1
Immunogen
Native Human Alpha Crystallin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01900 is reactive to CRYAA in Bovine, Human, Mouse, Rat
Applications
M01900 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
19.909kDa
Background of AlphaA Crystallin/CRYAA
The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Western blot analysis of CRYAA using anti-CRYAA antibody (M01900).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRYAA antigen affinity purified polyclonal antibody (Catalog # M01900) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for CRYAA.
Click image to see more details
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha A Crystallin Monoclonal Antibody, Clone 1H3.B8 (M01900) . Tissue: Neuroblastoma cell line (SK-N-BE) . Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody (M01900) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha A Crystallin Antibody. (D) Composite.
Protein Target Info & Infographic
Gene/Protein Information For CRYAA (Source: Uniprot.org, NCBI)
Gene Name
CRYAA
Full Name
Alpha-crystallin A chain
Weight
19.909kDa
Superfamily
small heat shock protein (HSP20) family
Alternative Names
AlphaA Crystallin; CRYA1; CRYA1crystallin, alpha-1; CRYAA; crystallin, alpha A; Heat shock protein beta-4; HSPB4; HSPB4alpha-crystallin A chain; human alphaA-crystallin (CRYA1), 10HspB4 CRYAA CRYA1, CTRCT9, HSPB4 crystallin alpha A alpha-crystallin A chain|crystallin, alpha-1|heat shock protein beta-4|human alphaA-crystallin (CRYA1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRYAA, check out the CRYAA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRYAA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alpha A Crystallin Monoclonal Antibody (M01900)
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Customer Q&As
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4 Customer Q&As for Anti-Alpha A Crystallin Monoclonal Antibody
Question
Would anti-Alpha A Crystallin Monoclonal antibody M01900 work on primate IF with spleen?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
Our lab technicians have not tested anti-Alpha A Crystallin Monoclonal antibody M01900 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Alpha A Crystallin Monoclonal antibody M01900 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate spleen in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-18
Question
Is a blocking peptide available for product anti-Alpha A Crystallin Monoclonal antibody (M01900)?
L. Walker
Verified customer
Asked: 2016-03-30
Answer
We do provide the blocking peptide for product anti-Alpha A Crystallin Monoclonal antibody (M01900). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-03-30
Question
I see that the anti-Alpha A Crystallin Monoclonal antibody M01900 works with WB, what is the protocol used to produce the result images on the product page?
R. Miller
Verified customer
Asked: 2015-08-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-08-20
Question
I have a question about product M01900, anti-Alpha A Crystallin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Wu
Verified customer
Asked: 2013-03-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01900 anti-Alpha A Crystallin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-03-08