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SKU:MA1104
Clonality:Monoclonal
Clone Number:EA-53
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Alpha-Actinin Antibody (Monoclonal, EA-53)
SKU/Catalog Number MA1104
Description Mouse IgG monoclonal antibody for alpha-Actinin, actinin, alpha 2 (ACTN2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Alpha-Actinin Antibody (Monoclonal, EA-53) EA-53 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1104)
Replacement Item This antibody may replace the following items: sc-130928|sc-15335|sc-166524|sc-17829|sc-7453|sc-7454-R from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Mouse, Rabbit, Rat
Predicted Species Monkey, Rabbit

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Rabbit skeletal alpha-actinin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number EA-53
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Actn2
Protein Name Alpha-actinin-2
Molecular Weight 103834 MW
Protein Function ACTN2: F-actin cross-linking protein which is thought to anchoractin to a variety of intracellular structures. This is a bundlingprotein.
Tissue Specificity ACTN2: Expressed in both skeletal and cardiac muscle.
Sequence Similarities Belongs to the alpha-actinin family.
Subcellular Localization ACTN2: Cytoplasm, myofibril, sarcomere, Z line
Actn2: Cytoplasm, myofibril, sarcomere, Z line
Uniprot ID D3ZCV0
Alternative Names Protein Actn2 ;RCG30552 ;Actn2 ;rCG_30552 ;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins| cardiovascular|heart|cardiac arrhythmias|contractility|contractile proteins|actins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Alpha-actinin-2

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin(alpha A) shares structural homology with spectrin and dystrophin.

Anti-Alpha-Actinin Antibody (Monoclonal, EA-53) Images

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Anti-Alpha-Actinin Antibody (Monoclonal, EA-53)
Figure 1. Western blot analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (MA1104).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat cardiac muscle tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: mouse cardiac muscle tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates,
Lane 5: human placenta tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Actinin antigen affinity purified monoclonal antibody (Catalog # MA1104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Alpha-Actinin at approximately 103KD. The expected band size for Alpha-Actinin is at 103KD.
Anti-Alpha-Actinin Antibody (Monoclonal, EA-53)
Figure 2. IHC analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (MA1104).
Alpha-Actinin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti- Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Anti-Alpha-Actinin Antibody (Monoclonal, EA-53)
Figure 3. IHC analysis of Alpha-Actinin using anti- Alpha-Actinin antibody (MA1104).
Alpha-Actinin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1.5μg/ml mouse anti- Alpha-Actinin Antibody (MA1104) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Publications

Xiao T, Zhang Y, Wang Y, Xu Y, Yu Z, Shen X. Food Chem Toxicol. 2013 Mar;53:364-70. Doi: 10.1016/J.Fct.2012.12.022. Epub 2012 Dec 22. Activation Of An Apoptotic Signal Transduction Pathway Involved In The Upregulation Of Calpain And Apoptosis-Indu...
Chen Sf, Rao Ly, Wei Tz, Xu Mg, Dong Zl. J Huazhong Univ Sci Technolog Med Sci. 2014 Dec;34(6):796-800. Doi: 10.1007/S11596-014-1355-4. Epub 2014 Dec 6. Expression Patterns Of Sarcomeric ??-Actin, ??-Actinin And Ucp2 In The Myocardium Of Kunming Mic...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.