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SKU:MA1107
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:DM1A
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A)
SKU/Catalog Number MA1107
Description Mouse IgG monoclonal antibody for alpha-Tubulin detection. Tested with WB, IHC-P in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A) DM1A (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1107)
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Human, Mouse, Rat
Predicted Species Chicken

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Microtubules from chicken embryo brain.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number DM1A
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TUBA1A
Protein Name Tubulin alpha-1A chain
Molecular Weight 50136 MW
Protein Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Tissue Specificity Expressed at a high level in fetal brain. .
Subcellular Localization Cytoplasm, cytoskeleton.
Uniprot ID Q71U36
Alternative Names Tubulin alpha-1A chain;Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;TUBA1A;TUBA3;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|microtubules|tubulin|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tubulin alpha-1A chain

Alpha-tubulin(b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.

Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A) Images

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Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A)
Anti-alpha-Tubulin antibody (monoclonal), MA1107, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Schwartz, K., & Bochkariov, D. (2017). Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity. Electrophoresis. Advance online publication. doi: 10.1002/elps.201700143
Chen Xh, Bao Yy, Zhou Sh, Wang Qy, Wei Y, Fan J. Mol Med Rep. 2013 Dec;8(6):1695-700. Doi: 10.3892/Mmr.2013.1740. Epub 2013 Oct 18. Glucose Transporter-1 Expression In Cd133+ Laryngeal Carcinoma Hep-2 Cells.
Zuo Q, Liu J, Zhang J, Wu M, Guo L, Liao W. Sci Rep. 2015 Jun 25;5:11634. Doi: 10.1038/Srep11634. Development Of Trastuzumab-Resistant Human Gastric Carcinoma Cell Lines And Mechanisms Of Drug Resistance.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tuba1a antibody, tuba4a antibody