|Product Name||Anti-AMD1 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for S-adenosylmethionine decarboxylase proenzyme(AMD1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-AMD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1070)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human AMD1(315-330aa FNDYNFVFTSFAKKQQ), identical to the related mouse and rat sequences.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-AMD1 antibody, PA1070, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-AMD1 antibody, PA1070, Western blotting
All lanes: Anti AMD1 (PA1070) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||S-adenosylmethionine decarboxylase proenzyme|
|Alternative Names||S-adenosylmethionine decarboxylase proenzyme;AdoMetDC;SAMDC;126.96.36.199;S-adenosylmethionine decarboxylase alpha chain;S-adenosylmethionine decarboxylase beta chain;AMD1;AMD;|
|Molecular Weight||38340 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Essential for biosynthesis of the polyamines spermidine and spermine. Promotes maintenance and self-renewal of embryonic stem cells, by maintaining spermine levels (By similarity). .|
|Research Areas||Cancer, Cell Biology, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Signal Transduction
*You can search these to find other products in these research areas.
|Background||S-adenosylmethionine decarboxylase(AdoMet-DC), also known as S-adenosylmethionine decarboxylase proenzyme(SAMDC), is a key enzyme in polyamine biosynthesis. It is localized to chromosome region 6q21-q22. SAMDC has an unusual distribution in polysomes from cells of T lymphocyte origin. It associates predominantly with monosomes and small polysomes with none located in the preribosomal or ribonucleoprotein pool. SAMDC is a critical regulatory enzyme of the polyamine synthetic pathway, and a well-studied drug target. Since SAMDC is a key regulatory enzyme in the synthesis of spermidine and spermine, the marked increase in SAMDC activity in the neonate and the sustained high enzyme levels throughout adulthood, imply a role for these polyamines in both development and mature brain function.|
Other Recommended Resources
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,