Product Info Summary
SKU: | M00542-2 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Androgen Receptor AR Rabbit Monoclonal Antibody
View all Androgen R/NR3C4 Antibodies
SKU/Catalog Number
M00542-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Androgen Receptor AR Rabbit Monoclonal Antibody catalog # M00542-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Androgen Receptor AR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00542-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CDF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Androgen Receptor
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00542-2 is reactive to AR in Human, Mouse, Rat
Applications
M00542-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
99.188kDa
Background of Androgen R/NR3C4
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Androgen Receptor expression in Lncap cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human prostate, using Androgen Receptor Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis of MCF7 cells, using Androgen Receptor Antibody .
Protein Target Info & Infographic
Gene/Protein Information For AR (Source: Uniprot.org, NCBI)
Gene Name
AR
Full Name
Androgen receptor
Weight
99.188kDa
Superfamily
nuclear hormone receptor family
Alternative Names
AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy AR AIS8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, AR androgen receptor androgen receptor|dihydrotestosterone receptor|nuclear receptor subfamily 3 group C member 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AR, check out the AR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Androgen Receptor AR Rabbit Monoclonal Antibody (M00542-2)
Hello CJ!
M00542-2 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone
Yangjing Capsule Ameliorates Spermatogenesis in Male Mice Exposed to Cyclophosphamide
Zhang Y, Shen Y, Cao B, Yan A, Ji H. Exp Ther Med. 2015 Mar;9(3):905-908. Epub 2014 Dec 19. Elevated Expression Levels Of Androgen Receptors And Matrix Metalloproteinase-2 And -9 In 30 Cases Of Hepatocellular Carcinoma Compared With Adjacent Tissu...
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