Data & Images
|Product Name||Anti-ACE/Cd143 Antibody|
|Description||Rabbit IgG polyclonal antibody for Angiotensin-converting enzyme(ACE) detection. Tested with WB in Human;Mouse.|
|Cite This Product||Anti-ACE/Cd143 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2196-2)|
|Replacement Item||This antibody may replace the following items: sc-12184|sc-376132|sc-20791|sc-12187|sc-374198|sc-23909|sc-69992|sc-69991|sc-69993|sc-69994|sc-23908|sc-271860 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Angiotensin Converting Enzyme 1(130-144aa ANLPLAKRQQYNALL), different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Angiotensin-converting enzyme|
|Molecular Weight||149715 MW|
|Protein Function||Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.|
|Tissue Specificity||Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .|
|Sequence Similarities||Belongs to the peptidase M2 family.|
|Subcellular Localization||Angiotensin-converting enzyme, soluble form: Secreted.|
|Alternative Names||Angiotensin-converting enzyme;ACE;3.2.1.-;184.108.40.206;Dipeptidyl carboxypeptidase I;Kininase II;CD143;Angiotensin-converting enzyme, soluble form;ACE;DCP, DCP1;|
|Research Areas|||cardiovascular|blood|serum proteins| stem cells|hematopoietic progenitors|surface molecules| signal transduction|metabolism|vitamins / minerals| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|aces| cardiovascular|vasculature|vasoconstriction| cancer|cancer metabolism|response to hypoxia| kits/ lysates/ other|elisa kits|cardiovascular elisa kits| metabolism|pathways and processes|cofactors, vitamins / minerals|metabolism processes||
Background for Angiotensin-converting enzyme
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ACE/Cd143 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ACE/Cd143 Antibody Images
Click the images to enlarge.
All lanes: Anti ACE (PA2196-2) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 150KD
Observed bind size: 150KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,