Overview
| Product Name |
Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody
See all Angiopoietin-like Protein 3/ANGPTL3 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
PA2075 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description |
Polyclonal antibody for Angiopoietin like Protein 3/ANGPTL3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. Angiopoietin like Protein 3/ANGPTL3 information: Molecular Weight: 53637 MW; Subcellular Localization: Secreted ; Tissue Specificity: Expressed principally in liver. Weakly expressed in kidney. |
| Cite This Product |
Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2075)
|
| Antibodies Validation |
Antibodies Validation Information
|
Similar Products From
Other Companies |
Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody may replace the following items: sc 68916|sc 68917|sc 83089|sc 365887. |
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Predicted Reactivity |
Hamster
*This antibody is predicted to react with the above species based on antigen
sequence similarities. Our Boster Guarantee covers the use of this product with the above species. |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of human ANGPTL3(32-48aa EPKSRFAMLDDVKILAN), identical to the related rat and mouse sequences. |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For ANGPTL3 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q9Y5C1 |
|---|
| NCBI Gene Id | 27329 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | ANGPTL3 |
|---|
| Protein Name | Angiopoietin-related protein 3 |
|---|
| Alternative Names | Angiopoietin-like Protein 3/ANGPTL3|AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2 |
|---|
| Molecular Weight | 53637 |
|---|
| Post Tranlational Modifications | Cleavage; Phosphorylation; Glycosylation; Oxidation |
|---|
| Subcellular Localization | Secreted. Cell projection, lamellipodium. Colocalized with HSPG2 and activated ITGB3 on podocytes. |
|---|
| Gene Location | 1p31.3, on Chromasome 1, gene sequence: NC_000001.11 (62597520..62606313) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ANGPTL3 (Source: Uniprot.org, NCBI)
| Protein Function | Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism (PubMed:11788823, PubMed:12909640, PubMed:23661675, PubMed:25495645). Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake (By similarity). Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1 (PubMed:12097324, PubMed:19318355, PubMed:20581395). Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids (PubMed:17110602, PubMed:19028676). Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol (PubMed:12565906). Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food; the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT (By similarity). Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR. May stimulate hypothalamic LPL activity (By similarity). |
|---|
| Tissue Specificity | Expressed principally in liver. Weakly expressed in kidney. Binds to adipocytes. Increased expression and colocalization with activated ITGB3 in glomeruli of patients with nephrotic syndrome showing effaced podocyte foot processes (at protein level). |
|---|
| Related Diseases | Obesity; Atherosclerosis; Dyslipidemias; Hyperlipidemia; Tumor Angiogenesis; Cardiovascular Diseases; Diabetes Mellitus; Insulin Resistance; Hypolipidaemia; Hypobetalipoproteinemias; Diabetes Mellitus, Non-insulin-dependent; Metabolic Syndrome X; Malignant Neoplasms; Tissue Adhesions; Liver Carcinoma; Familial Hypobetalipoproteinemia; Corneal Diseases; Coronary Heart Disease; Fatty Liver |
|---|
| Related Pathways | Angiogenesis; Secretion; Cell Adhesion; Transport; Pathogenesis; Cholesterol Homeostasis; Glycosylation; Rna Interference; Cell Migration; Immune Response; Protein Secretion; Liver Development; Protein Glycosylation; Reverse Transcription; Translation; Cholesterol Efflux; Lipid Homeostasis; Cholesterol Transport; Triglyceride Homeostasis; Cell Proliferation |
|---|
| Background | ANGPTL3(Angiopoietin-Like 3), also known as ANGPT5, is a protein which in humans is encoded by the ANGPTL3 gene. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. By radiation hybrid mapping and the use of surrounding genes, Conklin et al.(1999) mapped the ANGPTL3 gene to chromosome 1p31. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen(FBN)-like domain. Angptl3 also acts as dual inhibitor of lipoprotein lipase(LPL) and endothelial lipase(EL), and increases plasma triglyceride and HDL cholesterol in rodents. ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels. |
|---|
| Scientific References | PMID: 10644446 by Conklin D., et al. Identification of a mammalian angiopoietin-related protein expressed specifically in liver.
PMID: 11877390 by Camenisch G., et al. ANGPTL3 stimulates endothelial cell adhesion and migration via integrin alpha vbeta 3 and induces blood vessel formation in vivo. |
|---|
Our Boster Quality Guarantee for Anti-Angiopoietin-related protein 3 ANGPTL3 Antibody covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-ANGPTL3 antibody, PA2075, Western blotting
WB: A549 Cell Lysate
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 0
