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Anti-ANGPTL3 Antibody
Rabbit IgG polyclonal antibody for Angiopoietin-related protein 3(ANGPTL3) detection. Tested with WB in Human;Mouse;Rat.
| SKU | PA2075 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human, Mouse, Rat |
| Clonality | Polyclonal |
| Host | Rabbit |
| Ig Isotype | N/A |
| Applications | WB |
Overview
| Product Name | Anti-ANGPTL3 Antibody See all ANGPTL3 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | PA2075 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Polyclonal antibody for Angiopoietin like Protein 3/ANGPTL3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. Angiopoietin like Protein 3/ANGPTL3 information: Molecular Weight: 53637 MW; Subcellular Localization: Secreted ; Tissue Specificity: Expressed principally in liver. Weakly expressed in kidney. |
| Cite This Product | Anti-ANGPTL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2075) |
| Specificity | Anti-ANGPTL3 Antibody (PA2075) reacts with Human, Mouse, Rat ANGPTL3, in native form and recombinant. Superfamily members of ANGPTL3 are not reactive to PA2075. |
| Host | Rabbit |
| Contents/Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Form | Lyophilized |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ANGPTL3(32-48aa EPKSRFAMLDDVKILAN), identical to the related rat and mouse sequences. |
| Reactivity | Human, Mouse, Rat |
Assay Details
Our Boster Quality Guarantee for Anti-ANGPTL3 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-ANGPTL3 Antibody (PA2075).
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.Images And Assay Conditions
Anti-ANGPTL3 antibody, PA2075, Western blotting
WB: A549 Cell Lysate
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | Q9Y5C1 |
|---|---|
| Gene Name | ANGPTL3 |
| Protein Name | Angiopoietin-related protein 3 |
| Tissue Specificity | Expressed principally in liver. Weakly expressed in kidney. . |
| Alternative Names | Angiopoietin-related protein 3;Angiopoietin-5;ANG-5;Angiopoietin-like protein 3;ANGPTL3;ANGPT5;UNQ153/PRO179; |
| Subcellular Localization | Secreted . |
| Molecular Weight | 53637 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Research Areas | Human, Mouse, Rat *You can search these to find other products in these research areas. |
|---|---|
| Background | ANGPTL3(Angiopoietin-Like 3), also known as ANGPT5, is a protein which in humans is encoded by the ANGPTL3 gene. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. By radiation hybrid mapping and the use of surrounding genes, Conklin et al.(1999) mapped the ANGPTL3 gene to chromosome 1p31. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen(FBN)-like domain. Angptl3 also acts as dual inhibitor of lipoprotein lipase(LPL) and endothelial lipase(EL), and increases plasma triglyceride and HDL cholesterol in rodents. ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels. |
Other Recommended Resources
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Publishing with Anti-ANGPTL3 Antibody (PA2075)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]
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PA2075
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
Customer Q&As
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
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Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
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Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
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Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
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Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
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Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
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Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
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Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.