Anti-Annexin A2/ANXA2 Antibody

Annexin A2 antibody

Boster Bio Anti-Annexin A2/ANXA2 Antibody catalog # PA1348. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1348
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Annexin A2/ANXA2 Antibody

View all Annexin A2 Antibodies

SKU/Catalog Number

PA1348

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Annexin A2/ANXA2 Antibody catalog # PA1348. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Annexin A2/ANXA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1348)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Annexin A2, different from the rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1348 is reactive to ANXA2 in Human, Mouse, Rat

Applications

PA1348 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

38.604kDa

Background of Annexin A2

Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. The ANXA2 gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. This protein is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. Richard et al. (1994) presented an integration of the physical, expression, and genetic maps of human chromosome 15. They placed the ANXA2 gene in their region IV, i.e., 15q21-q22, thus confirming the previous localization.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ANXA2 (Source: Uniprot.org, NCBI)

Gene Name

ANXA2

Full Name

Annexin A2

Weight

38.604kDa

Superfamily

annexin family

Alternative Names

Annexin A2; Annexin II; annexin-2; ANX2; ANX2L4; ANX2L4LPC2; ANXA2; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; chromobindin-8; LIP2; LIP2PAP-IV; Lipocortin II; Lipocortin-2; LPC2D; LPC2DP36; p36; PAP-IV; Placental anticoagulant protein IV; Protein I ANXA2 ANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV annexin A2 annexin A2|annexin II|annexin-2|calpactin I heavy chain|calpactin I heavy polypeptide|calpactin-1 heavy chain|chromobindin 8|epididymis secretory protein Li 270|epididymis secretory sperm binding protein|lipocortin II|placental anticoagulant protein IV|protein I

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ANXA2, check out the ANXA2 Infographic

ANXA2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ANXA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1348 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhu J, Zou W, Jia G, Zhou H, Hu Y, Peng M, Chen H, Jin M. J Proteomics. 2012 Mar 16;75(6):1732-41. Doi: 10.1016/J.Jprot.2011.12.012. Epub 2011 Dec 20. Analysis Of Cellular Proteome Alterations In Porcine Alveolar Macrophage Cells Infected With 200...

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3 Customer Q&As for Anti-Annexin A2/ANXA2 Antibody

Question

My question regarding product PA1348, anti-Annexin A2/ANXA2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1348 anti-Annexin A2/ANXA2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-10

Question

We are currently using anti-Annexin A2/ANXA2 antibody PA1348 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

O. Wu

Verified customer

Asked: 2019-04-02

Answer

The anti-Annexin A2/ANXA2 antibody (PA1348) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-02

Question

Would anti-Annexin A2/ANXA2 antibody PA1348 work on monkey IHC with umbilical vein endothelial cell?

Verified Customer

Verified customer

Asked: 2018-11-21

Answer

Our lab technicians have not validated anti-Annexin A2/ANXA2 antibody PA1348 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Annexin A2/ANXA2 antibody PA1348 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey umbilical vein endothelial cell in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-21

Order DetailsPrice
PA1348

100μg

$370
PA1348-10ug

10μg sample (liquid)

$99
PA1348-Biotin

100 μg Biotin conjugated

$570
PA1348-Cy3

100 μg Cy3 conjugated

$570
PA1348-Dylight488

100 μg Dylight488 conjugated

$570
PA1348-Dylight550

100 μg Dylight550 conjugated

$570
PA1348-Dylight594

100 μg Dylight594 conjugated

$570
PA1348-FITC

100 μg FITC conjugated

$570
PA1348-HRP

100 μg HRP conjugated

$570
PA1348-APC

100 μg APC conjugated

$670
PA1348-PE

100 μg PE conjugated

$670
PA1348-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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