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SKU:PB9044
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $240.00
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Overview

Product Name Anti-Annexin V Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Cite This Product Anti-Annexin V Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9044)
Replacement Item This antibody may replace the following items: sc-166762|sc-1924|sc-1928|sc-1928-R|sc-1929|sc-271015|sc-271577|sc-28825|sc-28827|sc-32321|sc-393669|sc-74438|sc-8300 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ANXA5
Protein Name Annexin A5
Molecular Weight 35937 MW
Protein Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Sequence Similarities Belongs to the annexin family.
Uniprot ID P08758
Alternative Names Annexin A5;Anchorin CII;Annexin V;Annexin-5;Calphobindin I;CBP-I;Endonexin II;Lipocortin V;Placental anticoagulant protein 4;PP4;Placental anticoagulant protein I;PAP-I;Thromboplastin inhibitor;Vascular anticoagulant-alpha;VAC-alpha;ANXA5;ANX5, ENX2, PP4;
Research Areas |cell biology|apoptosis|annexin v reagents| cancer|invasion/microenvironment|detection kits|annexin v| cardiovascular|atherosclerosis| kits/ lysates/ other|elisa kits|apoptosis marker and proteins elisa kits|cell death|apoptotic markers|annexin v detection kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Annexin A5

Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Anti-Annexin V Picoband™ Antibody Images

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Anti-Annexin V Picoband™ Antibody
Anti-Annexin V Picoband antibody, PB9044-1.JPG
IHC(P): Human Intestinal Cancer Tissue
Anti-Annexin V Picoband™ Antibody
Anti-Annexin V Picoband antibody, PB9044-2.JPG
IHC(P): Human Mammary Cancer Tissue
Anti-Annexin V Picoband™ Antibody
Anti-Annexin V Picoband antibody, PB9044-3.JPG
IHC(P): Mouse Kidney Tissue
Anti-Annexin V Picoband™ Antibody
Anti-Annexin V Picoband antibody, PB9044-4.JPG
IHC(P): Rat Kidney Tissue
Anti-Annexin V Picoband™ Antibody
Anti-Annexin V Picoband antibody, PB9044-5.jpg
All lanes: Anti-Annexin V(PB9044) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at40ug
Lane 3: Rat Cardiac Muscle Tissue Lysate at 40ug
Lane 4: Rat Kidney Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: SGC Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
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Publications

Xiong Y, Ma Xy, Zhang Z, Shao Zj, Zhang Yy, Zhou Lm. Oncol Lett. 2013 Dec;6(6):1789-1793. Epub 2013 Oct 10. Apoptosis Induced By ??,??-Dimethylacrylshikonin Is Associated With Bcl-2 And Nf-??b In Human Breast Carcinoma Mcf-7 Cells.
Jin H, Liu T, Wang Wx, Xu Jh, Yang Pb, Lu Hx, Sun Qr, Hu Ht. Neurochem Int. 2010 Feb;56(3):417-23. Doi: 10.1016/J.Neuint.2009.11.015. Epub 2009 Nov 24. Protective Effects Of [Gly14]-Humanin On Beta-Amyloid-Induced Pc12 Cell Death By Preventing Mit...
Hu Xj, Dong Ng, Shi Jw, Deng C, Li Hd, Lu Cf. J Biomed Mater Res B Appl Biomater. 2014 Feb;102(2):322-36. Doi: 10.1002/Jbm.B.33010. Epub 2013 Sep 20. Evaluation Of A Novel Tetra-Functional Branched Poly(Ethylene Glycol) Crosslinker For Manufacture...
Ji N, Li J, Wei Z, Kong F, Jin H, Chen X, Li Y, Deng Y. Biomed Res Int. 2015;2015:308475. Doi: 10.1155/2015/308475. Epub 2015 Mar 19. Effect Of Celastrol On Growth Inhibition Of Prostate Cancer Cells Through The Regulation Of Herg Channel In Vitro.
Sun X, Kang H, Yao Y, Chen H, Sun L, An W, Jiang E, Wang S, Hu X. Anticancer Drugs. 2015 Jul;26(6):641-8. Doi: 10.1097/Cad.0000000000000229. Dehydrocostus Lactone Suppressed The Proliferation, Migration, And Invasion Of Colorectal Carcinoma Throug...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to annexin v antibody, annexin 5 antibody, annexin a5 antibody, annexin-5 antibody