|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-Annexin V Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-Annexin V Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9044)|
|Replacement Item||This antibody may replace the following items: sc-166762|sc-1924|sc-1928|sc-1928-R|sc-1929|sc-271015|sc-271577|sc-28825|sc-28827|sc-32321|sc-393669|sc-74438|sc-8300 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Annexin A5|
|Molecular Weight||35937 MW|
|Protein Function||This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.|
|Sequence Similarities||Belongs to the annexin family.|
|Alternative Names||Annexin A5;Anchorin CII;Annexin V;Annexin-5;Calphobindin I;CBP-I;Endonexin II;Lipocortin V;Placental anticoagulant protein 4;PP4;Placental anticoagulant protein I;PAP-I;Thromboplastin inhibitor;Vascular anticoagulant-alpha;VAC-alpha;ANXA5;ANX5, ENX2, PP4;|
|Research Areas|||cell biology|apoptosis|annexin v reagents| cancer|invasion/microenvironment|detection kits|annexin v| cardiovascular|atherosclerosis| kits/ lysates/ other|elisa kits|apoptosis marker and proteins elisa kits|cell death|apoptotic markers|annexin v detection kits||
Background for Annexin A5
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Annexin V Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Annexin V Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
IHC(P): Mouse Kidney Tissue
IHC(P): Rat Kidney Tissue
All lanes: Anti-Annexin V(PB9044) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at40ug
Lane 3: Rat Cardiac Muscle Tissue Lysate at 40ug
Lane 4: Rat Kidney Tissue Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: SGC Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,