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SKU:PB9118
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $240.00
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Overview

Product Name Anti-AP2 Alpha Picoband™ Antibody
SKU/Catalog Number PB9118
Description Rabbit IgG polyclonal antibody for Transcription factor AP-2-alpha(TFAP2A) detection. Tested with WB in Human;Rat.
Cite This Product Anti-AP2 Alpha Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9118)
Replacement Item This antibody may replace the following items: sc-12726|sc-12726-X|sc-184|sc-184-X|sc-25343|sc-25343-X|sc-53163|sc-53164|sc-55485|sc-6312|sc-6312-X|sc-70361|sc-8975|sc-8975-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human AP2 alpha recombinant protein (Position: M1-G166). Human AP2 alpha shares 98% amino acid (aa) sequence identity with both mouse and rat AP2 alpha.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TFAP2A
Protein Name Transcription factor AP-2-alpha
Molecular Weight 48062 MW
Protein Function Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. .
Sequence Similarities Belongs to the AP-2 family.
Subcellular Localization Nucleus .
Uniprot ID P05549
Alternative Names Transcription factor AP-2-alpha;AP2-alpha;AP-2 transcription factor;Activating enhancer-binding protein 2-alpha;Activator protein 2;AP-2;TFAP2A;AP2TF, TFAP2;
Research Areas |epigenetics and nuclear signaling|transcription|other factors| epigenetics and nuclear signaling|chip'ing antibodies|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Transcription factor AP-2-alpha

TFAP2A, also known as AP-2alpha or BOFS, is a protein that in humans is encoded by the TFAP2A gene. It is mapped to 6p24.3. The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. TFAP2A acts as a sequence-specific DNA-binding transcription factor recognizing and binding to the specific DNA sequence and recruiting transcription machinery. This gene is expressed in neural crest cell lineages with the highest levels of expression corresponding to early neural crest cells, suggesting that TFAP2A plays a role in their differentiation and development.

Anti-AP2 Alpha Picoband™ Antibody Images

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Anti-AP2 Alpha Picoband™ Antibody
Anti-AP2 alpha Picoband antibody, PB9118-1.jpg
All lanes: Anti AP2A (PB9118) at 0.5ug/ml
WB: Recombinant Human AP2A Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
Anti-AP2 Alpha Picoband™ Antibody
Anti-AP2 alpha Picoband antibody, PB9118-2.jpg
All lanes: Anti AP2A (PB9118) at 0.5ug/ml
WB: Rat Spleen Tissue Lysate at 50ug
Predicted bind size: 48KD
Observed bind size: 48KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is ap2-alpha antibody