Anti-APE1 Rabbit Monoclonal Antibody

APE antibody

Boster Bio Anti-APE1 Rabbit Monoclonal Antibody catalog # M00627-3. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00627-3
Size: 100 μl/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-APE1 Rabbit Monoclonal Antibody

View all APE Antibodies

SKU/Catalog Number

M00627-3

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-APE1 Rabbit Monoclonal Antibody catalog # M00627-3. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-APE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00627-3)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

17A44

Isotype

IgG

Immunogen

A synthesized peptide derived from human APE1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00627-3 is reactive to APEX1 in Human, Mouse, Rat

Applications

M00627-3 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

35.555kDa

Background of APE

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For APEX1 (Source: Uniprot.org, NCBI)

Gene Name

APEX1

Full Name

DNA-(apurinic or apyrimidinic site) lyase

Weight

35.555kDa

Superfamily

DNA repair enzymes AP/ExoA family

Alternative Names

AP endonuclease 1; APE; APE1; APE-1; APEAPEX nuclease (multifunctional DNA repair enzyme); APENAP endonuclease class I; APEX deoxyribonuclease (apurinic or apyrimidinic); APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease; APEX1; Apurinic-apyrimidinic endonuclease 1; APXAP lyase; DNA-(apurinic or apyrimidinic site) lyase; EC 3.1; EC 4.2.99.18; HAP1apurinic/apyrimidinic (abasic) endonuclease; multifunctional DNA repair enzyme; protein REF-1; redox factor 1; Redox factor-1; REF1 apurinic/apyrimidinic exonuclease; REF-1 APEX1 APE, APE1, APEN, APEX, APX, HAP1, REF1 apurinic/apyrimidinic endodeoxyribonuclease 1 DNA-(apurinic or apyrimidinic site) endonuclease|AP endonuclease class I|AP lyase|APEX nuclease (multifunctional DNA repair enzyme) 1|DNA-(apurinic or apyrimidinic site) lyase|apurinic-apyrimidinic endonuclease 1|apurinic/apyrimidinic (abasic) endonuclease|deoxyribonuclease (apurinic or apyrimidinic)|protein REF-1|redox factor-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on APEX1, check out the APEX1 Infographic

APEX1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for APEX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00627-3

100μL

$370
M00627-3-30ul

30μL sample

$99
M00627-3-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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Eddy test
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M00627-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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