|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-APEX2/Ape2 Antibody|
|Description||Rabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase 2(APEX2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-APEX2/Ape2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1495)|
|Replacement Item||This antibody may replace the following items: sc-130106|sc-66315|sc-66315-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human APEX2(504-518aa DPSSRCNFFLWSRPS), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||DNA-(apurinic or apyrimidinic site) lyase 2|
|Molecular Weight||57401 MW|
|Protein Function||Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. .|
|Tissue Specificity||Highly expressed in brain and kidney. Weakly expressed in the fetal brain. .|
|Sequence Similarities||Belongs to the DNA repair enzymes AP/ExoA family.|
|Subcellular Localization||Nucleus. Cytoplasm. Mitochondrion . Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.|
|Alternative Names||DNA-(apurinic or apyrimidinic site) lyase 2;3.1.-.-;22.214.171.124;AP endonuclease XTH2;APEX nuclease 2;APEX nuclease-like 2;Apurinic-apyrimidinic endonuclease 2;AP endonuclease 2;APEX2;APE2, APEXL2, XTH2;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|dna damage & repair|base excision repair||
Background for DNA-(apurinic or apyrimidinic site) lyase 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-APEX2/Ape2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-APEX2/Ape2 Antibody Images
Click the images to enlarge.
All lanes: Anti APEX2 (PA1495) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 57KD
Observed bind size: 57KD
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,