Anti-Apg7/ATG7 Antibody

ATG7 antibody

Boster Bio Anti-Apg7/ATG7 Antibody catalog # PA2261. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA2261
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, WB

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Product Name

Anti-Apg7/ATG7 Antibody

View all ATG7 Antibodies

SKU/Catalog Number

PA2261

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Apg7/ATG7 Antibody catalog # PA2261. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Apg7/ATG7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2261)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2261 is reactive to ATG7 in Human, Mouse, Rat

Applications

PA2261 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

78 kDa

Calculated molecular weight

77.96kDa

Background of ATG7

Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ATG7 (Source: Uniprot.org, NCBI)

Gene Name

ATG7

Full Name

Ubiquitin-like modifier-activating enzyme ATG7

Weight

77.96kDa

Superfamily

ATG7 family

Alternative Names

APG7 autophagy 7-like (S. cerevisiae); APG7; APG7L; APG7-LIKE; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7; Autophagy-related protein 7; DKFZp434N0735; GSA7; hAGP7; ubiquitin activating enzyme E1-like protein; Ubiquitin-activating enzyme E1-like protein; ubiquitin-like modifier-activating enzyme ATG7 ATG7 APG7-LIKE, APG7L, GSA7 autophagy related 7 ubiquitin-like modifier-activating enzyme ATG7|APG7 autophagy 7-like|ATG12-activating enzyme E1 ATG7|hAGP7|ubiquitin-activating enzyme E1-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATG7, check out the ATG7 Infographic

ATG7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATG7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA2261 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Erbin exerts a protective effect against inflammatory bowel disease by suppressing autophagic cell death

Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in the development of mouse placenta

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Order DetailsPrice
PA2261

100μg

$370
PA2261-10ug

10μg sample (liquid)

$99
PA2261-Biotin

100 μg Biotin conjugated

$570
PA2261-Cy3

100 μg Cy3 conjugated

$570
PA2261-Dylight488

100 μg Dylight488 conjugated

$570
PA2261-Dylight550

100 μg Dylight550 conjugated

$570
PA2261-Dylight594

100 μg Dylight594 conjugated

$570
PA2261-FITC

100 μg FITC conjugated

$570
PA2261-HRP

100 μg HRP conjugated

$570
PA2261-APC

100 μg APC conjugated

$670
PA2261-PE

100 μg PE conjugated

$670
PA2261-carrier-free

Carrier Free

$370

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