Data & Images
|Product Name||Anti-Apg7 Antibody|
|Description||Rabbit IgG polyclonal antibody for Ubiquitin-like modifier-activating enzyme ATG7(ATG7) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-Apg7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2261)|
|Replacement Item||This antibody may replace the following items: sc-160951|sc-376119|sc-377230|sc-390073|sc-160953|sc-98569|sc-166970|sc-160950|sc-398482|sc-160950|sc-160951|sc-160953|sc-166970|sc-376119|sc-377230|sc-390073|sc-398482|sc-98569 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7(652-669aa KVLDQYEREGFNFLAKVF), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Ubiquitin-like modifier-activating enzyme ATG7|
|Molecular Weight||77960 MW|
|Protein Function||E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. .|
|Tissue Specificity||Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .|
|Subcellular Localization||Cytoplasm . Preautophagosomal structure . Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .|
|Alternative Names||Ubiquitin-like modifier-activating enzyme ATG7;ATG12-activating enzyme E1 ATG7;Autophagy-related protein 7;APG7-like;hAGP7;Ubiquitin-activating enzyme E1-like protein;ATG7;APG7L;|
Background for Ubiquitin-like modifier-activating enzyme ATG7
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Apg7 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Apg7 Antibody Images
Click the images to enlarge.
All lanes: Anti-ATG7(PA2261) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 40ug
Lane 3: Human Placenta Tissue Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: 293T Whole Cell Lysate at 40ug
Lane 7: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,